Key features and details
- Rabbit polyclonal to BVR
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BVR antibody
See all BVR primary antibodies
DescriptionRabbit polyclonal to BVR
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
Protein previously labeled as Biliverdin Reductase.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192925 was affinity purified using an epitope specific to BVR immobilized on solid support.
Our Abpromise guarantee covers the use of ab192925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 33 kDa.|
FunctionReduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
PathwayPorphyrin-containing compound metabolism; protoheme degradation.
Involvement in diseaseHyperbiliverdinemia
Sequence similaritiesBelongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
- Information by UniProt
- BIEA_HUMAN antibody
- Biliverdin IX alpha reductase antibody
- Biliverdin reductase A antibody
Detection of BVR in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab192925 at 6 µg/reaction for IP and 1µg/ml for WB detection.
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-BVR antibody (ab192925) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes
ab192925 has not yet been referenced specifically in any publications.