Overview

  • Product name

    Anti-BZW1/Protein Orf antibody
    See all BZW1/Protein Orf primary antibodies
  • Description

    Rabbit polyclonal to BZW1/Protein Orf
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human BZW1/Protein Orf (C terminal). (BAD96965)

  • Positive control

    • Lymph nodes, HeLa , HepG2 and MCF7 cell lysate
  • General notes

     This product was previously labelled as BZW1

     

Properties

  • Form

    Lyophilised:Reconstitute in 200ul sterile H2O.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000. Predicted molecular weight: 48 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/50 - 1/200.

Target

  • Function

    Enhances histone H4 gene transcription but does not seem to bind DNA directly.
  • Sequence similarities

    Belongs to the BZW family.
    Contains 1 W2 domain.
  • Developmental stage

    Expressed in day 3 embryo.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic leucine zipper and W2 domain containing protein 1 antibody
    • Basic leucine zipper and W2 domain-containing protein 1 antibody
    • basic leucine-zipper protein BZAP45 antibody
    • BZAP45 antibody
    • bzw1 antibody
    • BZW1_HUMAN antibody
    • KIAA0005 antibody
    • Nbla10236 antibody
    • Protein Orf antibody
    • putative protein product of Nbla10236 antibody
    see all

Images

  • All lanes : Anti-BZW1/Protein Orf antibody (ab85090) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemical analysis of BZW1/Protein Orf expression in paraffin embedded formalin fixed lymph node section using 1/100 ab85090. note cytoplasmic staining.

References

This product has been referenced in:

  • Loughran G  et al. Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection. PLoS One 13:e0192648 (2018). Read more (PubMed: 29470543) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabead IgG
Sample
Human Tissue lysate - whole (brain)
Specification
brain
Total protein in input
50 µg

Abcam user community

Verified customer

Submitted Dec 06 2013

Application
ELISA
Sample
Human Cell (neuronal)
Specification
neuronal
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Type
Indirect

Abcam user community

Verified customer

Submitted Sep 05 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
75 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Sep 03 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
75 µg
Specification
neuronal
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Sep 03 2012

Answer

Thank you for your reply.   The antibody, ab85090 has the possibility to recognize BZW2, but the possibility is low. The antigen peptide is 15aa peptide and 9aa are the same in BZW2.   Please let me know if there is anything else I can help you with.    

Read More

Answer

Thank you for your reply. I am contacting the lab to see what the exact immunogen sequence ab85090 is. When I find out I will compare that to the BZW2 sequence and see what the level of homology is. If there is anything else I can do in the meantime, please let me kn

Read More

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