African green monkey Predicted to work with:
Mouse, Rat, Human
Synthetic phosphopeptide derived from human Abl1 around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.
extracts from COS7 cells, treated with Adriamycin
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 123 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
Involvement in disease
Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Isoform IB is myristoylated on Gly-2.
Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
ABL proto oncogene 1 non receptor tyrosine kinase antibody
bcr/c abl oncogene protein antibody
c ABL antibody
c abl oncogene 1 non receptor tyrosine kinase antibody
c abl oncogene 1 receptor tyrosine kinase antibody
c ABL1 antibody
Proto oncogene tyrosine protein kinase ABL1 antibody
Proto-oncogene c-Abl antibody
Tyrosine-protein kinase ABL1 antibody
v abl Abelson murine leukemia viral oncogene homolog 1 antibody
v abl antibody
Western blot - Anti-c Abl (phospho Y412) antibody (ab55284)
All lanes : Anti-c Abl (phospho Y412) antibody (ab55284) at 1/500 dilution
Lane 1 : extracts from COS7 cells, treated with Adriamycin (0.5ng/ml, 24 hours). Lane 2 : extracts from COS7 cells, treated with Adriamycin (0.5ng/ml, 24 hours); ab55284 pre-incubated with the immunising phosphopeptide.
Lysates/proteins at 5 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG (H+L)
Predicted band size: 123 kDa Observed band size: 123 kDa