Overview

  • Product name

    Anti-C-erb B-2 antibody [ERBB2/2453] - BSA and Azide free
    See all C-erb B-2 primary antibodies
  • Description

    Mouse monoclonal [ERBB2/2453] to C-erb B-2 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family.

  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human C-erb B-2 aa 311-462. The exact sequence is proprietary.
    Database link: P04626

  • Positive control

    • IHC-P: Human breast carcinoma tissue.
  • General notes

    ab237835 is a PBS-only buffer format of ab237993.

Properties

Applications

Our Abpromise guarantee covers the use of ab237835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Target

  • Relevance

    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
  • Database links

Images

  • This data was produced with ab237993, the same antibody in a different formulation with BSA and Azide.
    ab237993 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

References

ab237835 has not yet been referenced specifically in any publications.

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