Anti-c-Fos antibody [2H2] (ab208942)
Key features and details
- Mouse monoclonal [2H2] to c-Fos
- Suitable for: IHC-FrFl, IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-c-Fos antibody [2H2]
See all c-Fos primary antibodies -
Description
Mouse monoclonal [2H2] to c-Fos -
Host species
Mouse -
Tested applications
Suitable for: IHC-FrFl, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human c-Fos aa 1 to the C-terminus. purified from E. coli.
Database link: P01100 -
Positive control
- IHC-P: Rat hippocampus, rat brain, rat placenta, human cervix and human placenta. WB: Rat cortical neurons treated with membrane depolarization buffer. HeLa cell lysate (serum-starved and then stimulated with 20% Fetal Bovine Serum for 2 hours). ICC/IF: Rat brain neural cultures. HeLa cells, treated with 20% FBS for 2 hours following serum–starvation.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Affinity purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2H2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FrFl | (4) |
1/200.
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IHC-P | (6) |
1/1000.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 41 kDa.
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ICC/IF | (1) |
1/1000.
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Notes |
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IHC-FrFl
1/200. |
IHC-P
1/1000. |
WB
1/1000 - 1/2000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/1000. |
Target
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Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsPhosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 280795 Cow
- Entrez Gene: 2353 Human
- Entrez Gene: 14281 Mouse
- Entrez Gene: 100144486 Pig
- Entrez Gene: 314322 Rat
- Omim: 164810 Human
- SwissProt: O77628 Cow
- SwissProt: P01100 Human
see all -
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
Images
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All lanes : Anti-c-Fos antibody [2H2] (ab208942) at 1/1000 dilution
Lane 1 : Wild-type PC-12 Untreated Control NGF (0 ng/mL, 4 days) cell lysate
Lane 2 : Wild-type PC-12 Treated NGF (111 ng/mL, 4 days) cell lysate
Lane 3 : FOS knockout PC-12 Untreated Control NGF (0 ng/mL, 4 days) cell lysate
Lane 4 : FOS knockout PC-12 Treated NGF (111 ng/mL, 4 days) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45-55 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-c-Fos antibody [2H2] staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab208942 was shown to bind specifically to c-Fos. A band was observed at 45-55 kDa in treated wild-type PC-12 cell lysates with no signal observed at this size in FOS knockout cell line ab281615 (knockout cell lysate ab283111). Treatment of NGF has no observable affect on protein expression in this cell line. To generate this image, wild-type and FOS knockout PC-12 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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IHC image of c-Fos staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208942, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of c-Fos staining in a section of formalin-fixed paraffin-embedded normal human cervix* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208942, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of c-Fos staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208942, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
IHC image of c-Fos staining in a section of formalin-fixed paraffin-embedded normal rat placenta performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208942, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Section of rat hippocampus stained with ab208942, at a 1/200 dilution, in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
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All lanes : Anti-c-Fos antibody [2H2] (ab208942) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells in serum free media
Lane 2 : HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media
Lane 3 : Rat cortical neurons
Lane 4 : Rat cortical neurons treated with membrane depolarization buffer for 5hrs
Predicted band size: 41 kDaMultiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten.
The single band in red at 37 kDa represents GAPDH protein.
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All lanes : Anti-c-Fos antibody [2H2] (ab208942) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (serum-starved for 36 hours)
Lane 2 : HeLa cell lysate (serum-starved and then stimulated with 20% Fetal Bovine Serum for 2 hours)
Predicted band size: 41 kDaab208942 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum-starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with a monoclonal antibody against GAPDH, used as loading control.
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Rat brain neural cultures (left) and the same cells stimulated with membrane deplorization buffer for 5 hours (right).
This is a salt solution containing 170mM Potassium which depolarizes and stimulates gene expression in neuronal cells but has no effect on glia. Cultures were stained with ab208942 in green and rabbit anti-GFAP in red. Nuclear DNA is revealed in blue with the DNA stain DAPI.
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Top panel: HeLa (Human epithelial cell line from cervix adenocarcinoma) cells serum starved for 36 hours and then treated with PBS.
Bottom panel: HeLa cells, treated with 20% FBS for 2 hours following serum–starvation for 36 hours, labeling c-Fos using ab208942 at 1/1000 dilution (green).
Red: Vimentin counterstain. Nuclei labeled with DAPI (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (75)
ab208942 has been referenced in 75 publications.
- Zhu F et al. Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology. J Orthop Surg Res 18:21 (2023). PubMed: 36624462
- Su H et al. Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction. iScience 26:106338 (2023). IHC-P ; Mouse . PubMed: 36968090
- Li S et al. Sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA2b) mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain. Br J Pharmacol 179:2016-2036 (2022). PubMed: 34811737
- Sheng ZF et al. Impaired Kv7 channel activity in the central amygdala contributes to elevated sympathetic outflow in hypertension. Cardiovasc Res 118:585-596 (2022). PubMed: 33512443
- Augestad IL et al. Normalisation of glucose metabolism by exendin-4 in the chronic phase after stroke promotes functional recovery in male diabetic mice. Br J Pharmacol 179:677-694 (2022). PubMed: 33973246