Overview

  • Product name

    Anti-c-Fos antibody [7D6-A9-D4]
    See all c-Fos primary antibodies
  • Description

    Mouse monoclonal [7D6-A9-D4] to c-Fos
  • Host species

    Mouse
  • Specificity

    This antibody detects endogenous levels of c-Fos and does not cross-react with related proteins.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human, Monkey
  • Immunogen

    Recombinant fragment within Human c-Fos. The exact sequence is proprietary. Purified recombinant human c-Fos protein fragments expressed in E.coli. NM_005252.
    Database link: P01100

  • Positive control

    • HeLa, Cos7, HCT116 and C6 cell lysates.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 03/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team

Properties

Applications

Our Abpromise guarantee covers the use of ab184666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.

Target

  • Function

    Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
  • Sequence similarities

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications

    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • C FOS antibody
    • Cellular oncogene c fos antibody
    • Cellular oncogene fos antibody
    • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
    • FBJ murine osteosarcoma viral oncogene homolog antibody
    • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
    • FBJ Osteosarcoma Virus antibody
    • FOS antibody
    • FOS protein antibody
    • FOS_HUMAN antibody
    • G0 G1 switch regulatory protein 7 antibody
    • G0/G1 switch regulatory protein 7 antibody
    • G0S7 antibody
    • Oncogene FOS antibody
    • p55 antibody
    • proto oncogene c Fos antibody
    • Proto oncogene protein c fos antibody
    • Proto-oncogene c-Fos antibody
    • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/1000 dilution

    Lane 1 : COS7
    Lane 2 : HCT116

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HeLa cell lysate without TSA treatment
    Lane 2 : HeLa cell lysate with TSA treatment

    Predicted band size: 62 kDa



    This image was generated using the ascites version of the product.

  • All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with 50 uM TPA
    Lane 3 : HeLa cell lysate treated with 50 uM TSA

    Predicted band size: 62 kDa



    The lower panel is labeled with anti-GAPDH at 1/1000 dilution.

    This image was generated using the ascites version of the product.

  • All lanes : Anti-c-Fos antibody [7D6-A9-D4] (ab184666) at 1/500 dilution

    Lane 1 : HCT116 cell lysate
    Lane 2 : Cos7 cell lysate
    Lane 3 : C6 cell lysate

    Predicted band size: 62 kDa



    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Song J  et al. a-Linolenic Acid Inhibits Receptor Activator of NF-?B Ligand Induced (RANKL-Induced) Osteoclastogenesis and Prevents Inflammatory Bone Loss via Downregulation of Nuclear Factor-KappaB-Inducible Nitric Oxide Synthases (NF-?B-iNOS) Signaling Pathways. Med Sci Monit 23:5056-5069 (2017). Read more (PubMed: 29061958) »
  • Wang Z  et al. MicroRNA-101 has a suppressive role in osteosarcoma cells through the targeting of c-FOS. Exp Ther Med 11:1293-1299 (2016). Read more (PubMed: 27073439) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184666.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up