Overview

  • Product name

  • Description

    Goat polyclonal to c-Fos
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Pig, Carp
  • Immunogen

    Synthetic peptide, conjugated to Blue Carrier Protein by a Cysteine residue linker, corresponding to internal sequence amino acids 283-295 of Human c-Fos (NP_005243.1).

  • Positive control

    • WB: Recombinant Human c-Fos protein (ab56280), HeLa cell lysate. IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab156802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 41 kDa.

Target

  • Function

    Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
  • Sequence similarities

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications

    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • C FOS antibody
    • Cellular oncogene c fos antibody
    • Cellular oncogene fos antibody
    • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
    • FBJ murine osteosarcoma viral oncogene homolog antibody
    • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
    • FBJ Osteosarcoma Virus antibody
    • FOS antibody
    • FOS protein antibody
    • FOS_HUMAN antibody
    • G0 G1 switch regulatory protein 7 antibody
    • G0/G1 switch regulatory protein 7 antibody
    • G0S7 antibody
    • Oncogene FOS antibody
    • p55 antibody
    • proto oncogene c Fos antibody
    • Proto oncogene protein c fos antibody
    • Proto-oncogene c-Fos antibody
    • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
    see all

Images

  • Paraffin-embedded human pancreas tissue stained for c-Fos using ab156802 at 5 µg/ml in immunohistochemical analysis.

  • Anti-c-Fos antibody (ab156802) at 0.3 µg/ml + HeLa cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa



    Primary incubation of 1 hour.

References

This product has been referenced in:

  • Zhou M  et al. Cardiac Sympathetic Afferent Denervation Protects Against Ventricular Arrhythmias by Modulating Cardiac Sympathetic Nerve Activity During Acute Myocardial Infarction. Med Sci Monit 25:1984-1993 (2019). Read more (PubMed: 30877783) »
  • Dong P  et al. Musashi-2, a novel oncoprotein promoting cervical cancer cell growth and invasion, is negatively regulated by p53-induced miR-143 and miR-107 activation. J Exp Clin Cancer Res 36:150 (2017). Read more (PubMed: 29073938) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - Triton
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Khoa Do

Verified customer

Submitted Apr 08 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Jennifer Remus

Verified customer

Submitted Apr 22 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - TritonX, Retrieval
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample
Fish Tissue sections (brain, preoptic area)
Specification
brain, preoptic area
Permeabilization
Yes - Triton-100, Tween
Fixative
Formaldehyde

Dr. Cynthia O'Rourke

Verified customer

Submitted Aug 29 2014

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