Overview

  • Product name
  • Description
    Sheep polyclonal to c-Fos
  • Host species
    Sheep
  • Specificity
    Extensive immunohistochemical analysis shows that it has high specificity to cFOS.
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide corresponding to Human c-Fos aa 2-17. The peptide was conjugated to diphtheria toxoid cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).
    Sequence:

    H-MFSGFNADYEASSSRC

  • General notes
    This antibody is very useful for double labelling in immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab6167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
  • Sequence similarities
    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications
    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • C FOS antibody
    • Cellular oncogene c fos antibody
    • Cellular oncogene fos antibody
    • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
    • FBJ murine osteosarcoma viral oncogene homolog antibody
    • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
    • FBJ Osteosarcoma Virus antibody
    • FOS antibody
    • FOS protein antibody
    • FOS_HUMAN antibody
    • G0 G1 switch regulatory protein 7 antibody
    • G0/G1 switch regulatory protein 7 antibody
    • G0S7 antibody
    • Oncogene FOS antibody
    • p55 antibody
    • proto oncogene c Fos antibody
    • Proto oncogene protein c fos antibody
    • Proto-oncogene c-Fos antibody
    • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
    see all

References

This product has been referenced in:
  • Hughes S  et al. Characterisation of light responses in the retina of mice lacking principle components of rod, cone and melanopsin phototransduction signalling pathways. Sci Rep 6:28086 (2016). Read more (PubMed: 27301998) »
See 1 Publication for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (intestine)
Permeabilization
Yes - 0.3% tritonX/PBS
Specification
intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 28 2018

Answer

Thank you for your inquiry.

We do not carry any anti-c-Fos antibodies that are known to work in rabbit. However, we have a few polyclonal antibodies that aren't raised in rabbit which may work due to high sequence homology with rabbit.

Goat polyclonal, ab87655, shows 96% homology with rabbit. https://www.abcam.com/c-Fos-antibody-ab87655.html

Goat polyclonal, ab87655, shows 96% homology with rabbit. https://www.abcam.com/c-Fos-antibody-ab62147.html

Chicken polyclonal, ab14285, shows 93% homology with rabbit. https://www.abcam.com/c-Fos-antibody-ab14285.html

Sheep polyclonal, ab6167, shows 94% homology with rabbt. https://www.abcam.com/c-Fos-antibody-ab6167.html

To our knowledge, none of these antibodies have been tested in rabbit. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing a product and specify the catalogue number.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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Answer

DISCOUNT CODE: XXXXXXX Expiration date: [15/03/2012] I am very pleased to hear you would like to accept our offer and test ab6167 in FrFl. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for FrFl and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your interest. I can certainly offer a discount code for testing ab6167 for FrFl, this antibody has only been tested in IHC-Fr (mouse and human). Could you please pass the customer's name, e-mail address and institute? I look forward to hearing from you soon.

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Question
Answer

Thank you for your enquiry. All the information we have available concerning this antibody is that listed on our datasheet. We are not aware of there being publications featuring this particular antibody. All we have are general background references. We will update our datasheet as soon as we get more information.

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Answer

Thank you for your enquiry. Although the caps are different, the antibody is from the same batch of animal. We are not sure why there is difference to the second vial. We will send you a replacement vial in the next order.

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Answer

Almost (99.9%) certainly yes because it is a polyclonal which recgnises multiple epitopes on the highly conservec c-fos across mammals.

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Answer

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

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Answer

There is nothing special about this antibody regarding its use in immunohistochemistry. The antibody can be used with a routine 4% formaldehyde fixation (we usually add 15% saturated picric acid) on cryostat sections. Thus, use a standard IHC protocol.

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Answer

Apologies but the data protection act prevents us from giving email addresses of our customers without their permission.

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1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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