Key features and details
- Rabbit polyclonal to c-Fos (phospho T232)
- Suitable for: IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Product nameAnti-c-Fos (phospho T232) antibody
See all c-Fos primary antibodies
DescriptionRabbit polyclonal to c-Fos (phospho T232)
Specificityab193365 detects endogenous levels of c-FOS only when phosphorylated at threonine 232.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human c-Fos (phospho T232) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P01100
- Human breast carcinoma, COS7 cell extracts
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193365 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab193365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 41 kDa.|
FunctionNuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Sequence similaritiesBelongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.
modificationsPhosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling c-FOS with ab193365 at 1/50 dilution. Right panel was preincubated with blocking peptide.
All lanes : Anti-c-Fos (phospho T232) antibody (ab193365) at 1/500 dilution
Lane 1 : COS7 cell extracts with Antigen specific peptide
Lane 2 : COS7 cell extracts
Predicted band size: 41 kDa
ab193365 has not yet been referenced specifically in any publications.