Anti-c-Fos (phospho T232) antibody (ab43175)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-c-Fos (phospho T232) antibody
See all c-Fos primary antibodies -
Description
Rabbit polyclonal to c-Fos (phospho T232) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Pig, Chimpanzee
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human c-Fos, phosphorylated at T232.
Read Abcam's proprietary immunogen policy (Peptide available as ab43652.) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: A431 - EGF treated, HeLa, Hela - Bleomycin Treated (40U/ml), Hela - Hydroxyurea Treated (48hr, 2uM). This antibody also gave a positive signal against the Full length Recombinant FOS protein (Tagged).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Control Peptide
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab43175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsPhosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2353 Human
- Entrez Gene: 100144486 Pig
- Omim: 164810 Human
- SwissProt: P01100 Human
- SwissProt: O97930 Pig
- SwissProt: O02761 Sheep
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
Images
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Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)ab43175 detects a 95 kDa band in western blot. This is consistent with results obtained by other commercially available antibodies to this target, as c-Fos has been reported to dimerise with the c-Jun protein hence migration at a higher molecular weight than predicted. -
Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)Anti-c-Fos (phospho T232) antibody (ab43175) at 1 µg/ml +Recombinant Human c-Fos protein (ab56280) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
ab56280, c-Fos protein (Tagged), has a predicted molecular weight of 78 kDa in SDS-PAGE.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Araújo FC et al. Expression of the proto-oncogene c-fos and the immunolocalization of c-fos, phosphorylated c-fos and estrogen receptor beta in the human testis. Histol Histopathol 24:1515-22 (2009). IHC-P ; Human . Read more (PubMed: 19795350) »
- Morsch DM et al. c-fos gene and protein expression in pelvic endometriosis: a local marker of estrogen action. J Mol Histol 40:53-8 (2009). IHC-P ; Human . Read more (PubMed: 19199093) »
