Anti-c-Fos (phospho T232) antibody (ab43175)

Submit a review Submit a question References (2)
Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)
  • Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)

Key features and details

  • Rabbit polyclonal to c-Fos (phospho T232)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-c-Fos (phospho T232) antibody
    See all c-Fos primary antibodies

  • Description

    Rabbit polyclonal to c-Fos (phospho T232)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human c-Fos, phosphorylated at T232.

    Read Abcam's proprietary immunogen policy (Peptide available as ab43652.)

  • Positive control

    • This antibody gave a positive signal in the following human whole cell lysates: A431 - EGF treated, HeLa, Hela - Bleomycin Treated (40U/ml), Hela - Hydroxyurea Treated (48hr, 2uM). This antibody also gave a positive signal against the Full length Recombinant FOS protein (Tagged).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab43175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Sheep
Pig
Chimpanzee
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 41 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 41 kDa).

Target

  • Function

    Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

  • Sequence similarities

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.

  • Post-translational
    modifications

    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • C FOS antibody
      • Cellular oncogene c fos antibody
      • Cellular oncogene fos antibody
      • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
      • FBJ murine osteosarcoma viral oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ Osteosarcoma Virus antibody
      • FOS antibody
      • FOS protein antibody
      • FOS_HUMAN antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0/G1 switch regulatory protein 7 antibody
      • G0S7 antibody
      • Oncogene FOS antibody
      • p55 antibody
      • proto oncogene c Fos antibody
      • Proto oncogene protein c fos antibody
      • Proto-oncogene c-Fos antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      see all

    Images

    • Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)
      Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)
      ab43175 detects a 95 kDa band in western blot. This is consistent with results obtained by other commercially available antibodies to this target, as c-Fos has been reported to dimerise with the c-Jun protein hence migration at a higher molecular weight than predicted.
    • Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)
      Western blot - Anti-c-Fos (phospho T232) antibody (ab43175)
      Anti-c-Fos (phospho T232) antibody (ab43175) at 1 µg/ml + Recombinant Human c-Fos protein (ab56280) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?



      ab56280, c-Fos protein (Tagged), has a predicted molecular weight of 78 kDa in SDS-PAGE.

    References (2)

    Publishing research using ab43175? Please let us know so that we can cite the reference in this datasheet.

    ab43175 has been referenced in 2 publications.

    • Araújo FC  et al. Expression of the proto-oncogene c-fos and the immunolocalization of c-fos, phosphorylated c-fos and estrogen receptor beta in the human testis. Histol Histopathol 24:1515-22 (2009). IHC-P ; Human . PubMed: 19795350
    • Morsch DM  et al. c-fos gene and protein expression in pelvic endometriosis: a local marker of estrogen action. J Mol Histol 40:53-8 (2009). IHC-P ; Human . PubMed: 19199093

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab43175.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com