Anti-c-IAP1 and c-IAP2 antibody (ab25939)
Key features and details
- Rabbit polyclonal to c-IAP1 and c-IAP2
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-c-IAP1 and c-IAP2 antibody -
Description
Rabbit polyclonal to c-IAP1 and c-IAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
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Positive control
- WB: HEK293, K562, A549. Human thymus, lung, testis, and small intestine lysates. Rat brain lysate. IHC-P: Human lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
cIAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
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| IHC-P |
Use a concentration of 10 - 20 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa. |
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IHC-P
Use a concentration of 10 - 20 µg/ml. |
Target
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Database links
- SwissProt: Q13489 Human
- SwissProt: Q13490 Human
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Alternative names
- cellular inhibitor of apoptosis protein 1 antibody
- cellular inhibitor of apoptosis protein 2 antibody
- cellular inhibitor of apoptosis proteins 1 and 2 antibody
Images
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 1 : Human Thymus
Lane 2 : Human Lung
Lane 3 : Human Testis
Lane 4 : Human Small Intestine
Lane 5 : Rat Brain
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 1 : HEK293 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : A549 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : Human lung lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP Conjugate at 1/10000 dilutionBlocking buffer: 5% NFDM/TBST.
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : Human lung lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Immunohistochemical analysis of paraffin-embedded human lung tissue using ab25939 at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with ab25939 overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) was used as a secondary at 1/250 dilution. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Immunohistochemical analysis of 4% paraformaldehyde-fixed human lung tissue labelling cIAP using ab25939 at 20 µg/ml, followed by Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab25939 has been referenced in 7 publications.
- Yan H et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). PubMed: 29207128
- Zhang Y et al. Novel ADAM-17 inhibitor ZLDI-8 enhances the in vitro and in vivo chemotherapeutic effects of Sorafenib on hepatocellular carcinoma cells. Cell Death Dis 9:743 (2018). PubMed: 29970890
- Zhang HP et al. Oridonin synergistically enhances JQ1-triggered apoptosis in hepatocellular cancer cells through mitochondrial pathway. Oncotarget 8:106833-106843 (2017). PubMed: 29290992
- Woo M et al. Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Pract 11:445-451 (2017). PubMed: 29209454
- Kobayashi H et al. Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. Nat Commun 7:13336 (2016). PubMed: 27830706
- Gualco E et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P ; Mouse . PubMed: 19834489
- Zhao TT et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB ; Human . PubMed: 18846111