Anti-c-IAP1 and c-IAP2 antibody (ab25939)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-c-IAP1 and c-IAP2 antibody
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DescriptionRabbit polyclonal to c-IAP1 and c-IAP2
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Host speciesRabbit
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SpecificityThis antibody detects both c-IAP1 and c-IAP2.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
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Positive control
- A549 whole cell lysate (ab7910) can be used as a positive control in WB. Human lung cell lysate can be used as positive control.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notescIAP Antibody is affinity chromatography purified via peptide column.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab25939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. |
Images
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : human lung lysate -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : Human lung lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.
Protocols
References
This product has been referenced in:
- Yan H et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). Read more (PubMed: 29207128) »
- Zhang Y et al. Novel ADAM-17 inhibitor ZLDI-8 enhances the in vitro and in vivo chemotherapeutic effects of Sorafenib on hepatocellular carcinoma cells. Cell Death Dis 9:743 (2018). Read more (PubMed: 29970890) »
