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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-c-IAP1 and c-IAP2 antibody (ab25939)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
  • Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)

Key features and details

  • Rabbit polyclonal to c-IAP1 and c-IAP2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-c-IAP1 and c-IAP2 antibody
  • Description

    Rabbit polyclonal to c-IAP1 and c-IAP2
  • Host species

    Rabbit
  • Specificity

    This antibody detects both c-IAP1 and c-IAP2.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1

  • Positive control

    • A549 whole cell lysate (ab7910) can be used as a positive control in WB. Human lung cell lysate can be used as positive control.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    cIAP Antibody is affinity chromatography purified via peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cancer Metabolism
    • Transcriptional control
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P
Use a concentration of 10 µg/ml.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P
Use a concentration of 10 µg/ml.

Target

  • Database links

    • SwissProt: Q13489 Human
    • SwissProt: Q13490 Human
    • Alternative names

      • cellular inhibitor of apoptosis protein 1 antibody
      • cellular inhibitor of apoptosis protein 2 antibody
      • cellular inhibitor of apoptosis proteins 1 and 2 antibody

    Images

    • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml

      Lane 1 : Human Thymus
      Lane 2 : Human Lung
      Lane 3 : Human Testis
      Lane 4 : Human Small Intestine
      Lane 5 : Rat Brain

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml

      Lane 1 : 293 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : A549 cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
      Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
      Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

      All lanes : human lung lysate
    • Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
      Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
      Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml

      All lanes : Human lung lysate
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)

      ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC 

    • Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)
      Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab25939? Please let us know so that we can cite the reference in this datasheet.

    ab25939 has been referenced in 7 publications.

    • Yan H  et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). PubMed: 29207128
    • Zhang Y  et al. Novel ADAM-17 inhibitor ZLDI-8 enhances the in vitro and in vivo chemotherapeutic effects of Sorafenib on hepatocellular carcinoma cells. Cell Death Dis 9:743 (2018). PubMed: 29970890
    • Zhang HP  et al. Oridonin synergistically enhances JQ1-triggered apoptosis in hepatocellular cancer cells through mitochondrial pathway. Oncotarget 8:106833-106843 (2017). PubMed: 29290992
    • Woo M  et al. Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Pract 11:445-451 (2017). PubMed: 29209454
    • Kobayashi H  et al. Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. Nat Commun 7:13336 (2016). PubMed: 27830706
    • Gualco E  et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P ; Mouse . PubMed: 19834489
    • Zhao TT  et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB ; Human . PubMed: 18846111

    Customer reviews and Q&As

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    Western blot abreview for Anti-cIAP1 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    25 µg
    Specification
    HEK293
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

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    Verified customer

    Submitted Jul 23 2012

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