Anti-c-IAP1 and c-IAP2 antibody (ab25939)
Key features and details
- Rabbit polyclonal to c-IAP1 and c-IAP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-c-IAP1 and c-IAP2 antibody -
Description
Rabbit polyclonal to c-IAP1 and c-IAP2 -
Host species
Rabbit -
Specificity
This antibody detects both c-IAP1 and c-IAP2. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
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Positive control
- A549 whole cell lysate (ab7910) can be used as a positive control in WB. Human lung cell lysate can be used as positive control.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
cIAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. |
Target
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Database links
- SwissProt: Q13489 Human
- SwissProt: Q13490 Human
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Alternative names
- cellular inhibitor of apoptosis protein 1 antibody
- cellular inhibitor of apoptosis protein 2 antibody
- cellular inhibitor of apoptosis proteins 1 and 2 antibody
Images
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 1 : Human Thymus
Lane 2 : Human Lung
Lane 3 : Human Testis
Lane 4 : Human Small Intestine
Lane 5 : Rat Brain
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 1 : 293 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : A549 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : human lung lysate -
Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : Human lung lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab25939 has been referenced in 7 publications.
- Yan H et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). PubMed: 29207128
- Zhang Y et al. Novel ADAM-17 inhibitor ZLDI-8 enhances the in vitro and in vivo chemotherapeutic effects of Sorafenib on hepatocellular carcinoma cells. Cell Death Dis 9:743 (2018). PubMed: 29970890
- Zhang HP et al. Oridonin synergistically enhances JQ1-triggered apoptosis in hepatocellular cancer cells through mitochondrial pathway. Oncotarget 8:106833-106843 (2017). PubMed: 29290992
- Woo M et al. Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis. Nutr Res Pract 11:445-451 (2017). PubMed: 29209454
- Kobayashi H et al. Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. Nat Commun 7:13336 (2016). PubMed: 27830706
- Gualco E et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P ; Mouse . PubMed: 19834489
- Zhao TT et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB ; Human . PubMed: 18846111