Anti-c-Jun antibody (ab131497)
Key features and details
- Rabbit polyclonal to c-Jun
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-c-Jun antibody
See all c-Jun primary antibodies -
Description
Rabbit polyclonal to c-Jun -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide from around amino acids 88-93 (
TTTPT
) of Human c-Jun (NP_002219.1) -
Positive control
- HeLa cells, 293T cell lysate, Human breast carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 35 kDa.
|
|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
Target
-
Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
-
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
Images
-
Western blot - Anti-c-Jun antibody (ab131497)All lanes : Anti-c-Jun antibody (ab131497) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : HeLa cell extract
Predicted band size: 35 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody (ab131497)Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131497 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (ab131497)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131497 at 1/50 dilution (left) and ab131497 preincubated with blocking peptide (right).
Protocols
Datasheets and documents
References (0)
ab131497 has not yet been referenced specifically in any publications.
