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    c-jun-antibody-ab151780.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-c-Jun antibody (ab151780)

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Western blot - Anti-c-Jun antibody (ab151780)

    Key features and details

    • Rabbit polyclonal to c-Jun
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant Human c-Jun protein (ab84134)

    View more associated products

    Overview

    • Product name

      Anti-c-Jun antibody
      See all c-Jun primary antibodies
    • Description

      Rabbit polyclonal to c-Jun
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse c-Jun aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
      Database link: P05627

    • Positive control

      • This antibody gave a positive signal in Raw264.7 and NIH3T3 whole cell lysate

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • Leucine Zipper
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • SMADs
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • SMADs
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Immunology
      • Innate Immunity
      • TLR Signaling
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Phosphoprotein ELISA kits

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human c-Jun protein (ab84134)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab151780 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Rat
    Rabbit
    Cow
    Human
    Pig
    Chimpanzee
    Macaque monkey
    Gorilla
    Orangutan
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).

    Target

    • Function

      Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
    • Sequence similarities

      Belongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      modifications

      Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
      Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
      Acetylated at Lys-271 by EP300.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P05412 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 280831 Cow
      • Entrez Gene: 3725 Human
      • Entrez Gene: 16476 Mouse
      • Entrez Gene: 396913 Pig
      • Entrez Gene: 24516 Rat
      • Omim: 165160 Human
      • SwissProt: P05412 Human
      • SwissProt: P05627 Mouse
      • SwissProt: P56432 Pig
      • SwissProt: P17325 Rat
      • Unigene: 696684 Human
      • Unigene: 275071 Mouse
      • Unigene: 93714 Rat
      see all
    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • AP-1 antibody
      • AP1 antibody
      • cJun antibody
      • Enhancer Binding Protein AP1 antibody
      • Jun Activation Domain Binding Protein antibody
      • JUN antibody
      • Jun oncogene antibody
      • JUN protein antibody
      • Jun proto oncogene antibody
      • JUN_HUMAN antibody
      • JUNC antibody
      • Oncogene JUN antibody
      • p39 antibody
      • Proto oncogene c jun antibody
      • Proto oncogene cJun antibody
      • Proto-oncogene c-jun antibody
      • Transcription Factor AP 1 antibody
      • Transcription factor AP-1 antibody
      • Transcription Factor AP1 antibody
      • V jun avian sarcoma virus 17 oncogene homolog antibody
      • V jun sarcoma virus 17 oncogene homolog (avian) antibody
      • V jun sarcoma virus 17 oncogene homolog antibody
      • V-jun avian sarcoma virus 17 oncogene homolog antibody
      • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all

    Images

    • Western blot - Anti-c-Jun antibody (ab151780)
      Western blot - Anti-c-Jun antibody (ab151780)
      All lanes : Anti-c-Jun antibody (ab151780) at 1 µg/ml (Milk blocking 3%)

      Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
      Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 90 kDa (possible non-specific binding)


      Exposure time: 20 minutes


      The predicted molecular weight of c-jun is 36 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa.

      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151780 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151780? Please let us know so that we can cite the reference in this datasheet.

    ab151780 has not yet been referenced specifically in any publications.

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