Key features and details
- Rabbit polyclonal to c-Jun
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-c-Jun antibody
See all c-Jun primary antibodies
DescriptionRabbit polyclonal to c-Jun
Specificityab31367 detects endogenous levels of c-Jun protein around Serine 73.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human c-Jun. Range of aa 40-89 Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.
Database link: P05412
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Corresponding phospho antibody
Our Abpromise guarantee covers the use of ab31367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling c-Jun with ab31367 at 1/100. Left: c-Jun antibody, Right: Preincubated with synthesized peptide.
All lanes : Anti-c-Jun antibody (ab31367) at 1/1000 dilution
Lane 1 : HeLa cells lysate, preincubated with immunogen (synthetic non-phosphopeptide)
Lane 2 : HeLa cells lysate
Predicted band size: 36 kDa
ab31367 has been referenced in 3 publications.
- Yuan D et al. Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS. Cancer Cell 31:771-789.e6 (2017). PubMed: 28609656
- Winter C et al. Google goes cancer: improving outcome prediction for cancer patients by network-based ranking of marker genes. PLoS Comput Biol 8:e1002511 (2012). IHC-Fr ; Human . PubMed: 22615549
- Samson WK et al. Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem 283:31949-59 (2008). IHC-P ; Mouse . PubMed: 18753129