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    c-jun-antibody-ab5795.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-c-Jun antibody (ab5795)

  • Datasheet
Reviews (1)Q&A (2)

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Abpromise

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Key features and details

  • Rabbit polyclonal to c-Jun
  • Reacts with: Mouse
  • Isotype: IgG

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Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Human JUN (c-Jun) knockout A431 cell line (ab276082)
Peptide
KAT13D / CLOCK peptide (ab5854)

View more associated products

Overview

  • Product name

    Anti-c-Jun antibody
    See all c-Jun primary antibodies
  • Description

    Rabbit polyclonal to c-Jun
  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse c-Jun aa 91-105.
    Sequence:

    TPTPTQFLCPKNVTD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • NIH 3T3 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Phosphoprotein ELISA kits

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human c-Jun protein (ab84134)

Target

  • Function

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similarities

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P05412 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 424673 Chicken
    • Entrez Gene: 280831 Cow
    • Entrez Gene: 3725 Human
    • Entrez Gene: 16476 Mouse
    • Entrez Gene: 396913 Pig
    • Entrez Gene: 24516 Rat
    • Entrez Gene: 443219 Sheep
    • Entrez Gene: 399400 Xenopus laevis
    • Omim: 165160 Human
    • SwissProt: P18870 Chicken
    • SwissProt: P05412 Human
    • SwissProt: P05627 Mouse
    • SwissProt: P56432 Pig
    • SwissProt: P17325 Rat
    • Unigene: 696684 Human
    • Unigene: 275071 Mouse
    • Unigene: 93714 Rat
    see all
  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP-1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab5795? Please let us know so that we can cite the reference in this datasheet.

    ab5795 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    ChIP abreview for Anti-c-Jun antibody

    Average
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Mouse Tissue lysate - whole (skin epidermis)
    Specification
    skin epidermis
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Semiquantitative PCR
    Positive control
    Untreated and TPA treated samples were used. Only in the case of TPA treated sample, a specific signal was obtained. Another c-jun antibody from a different company, which we had tested earlier and obtained positive result, was also included in parallel. In the case of both Abcam and the other companies antibodies, specific signal was obtained only when the skin was treated with TPA. However, the other companies antibody was more efficient in immunoprecipitation in comparison to Abcam antibody.
    Negative control
    A region corresponding to the coding sequence of the gene, which does not reveal any c-jun binding site by bioinformatics analysis, was used for PCR amplification. However, no amplification was observed.
    Also there was no binding in the absence of TPA as mentioned above.
    Read More

    Dr. milan surjit

    Verified customer

    Submitted Sep 21 2007

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