Product nameAnti-c-Jun antibody - ChIP Grade
See all c-Jun primary antibodies
DescriptionRabbit polyclonal to c-Jun - ChIP Grade
SpecificityAntibody detects endogenous levels of c-Jun protein around Serine 243.
Tested applicationsSuitable for: ChIP, ICC/IF, IHC-P, IP, WB, ELISA, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human, African green monkey
Predicted to work with: Chicken, Cow, Pig
- ChIP: Human endothelial cells (EA.hy926). IHC-P: Human breast carcinoma tissue. ICC/IF: HepG2 cells. WB: Extracts of HeLa cells. Recombinant Human c-Jun protein. Flow Cytometry: Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ChIP Related Products
Corresponding phospho antibody
Our Abpromise guarantee covers the use of ab31419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100.|
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
ChIP analysis using ab31419 binding c-Jun in human endothelial cells (EA.hy926). Cells were cross-linked for 10 minutes with formaldehyde then incubated with undiluted primary antibody for 10 hours at 4°C in 1x ChIP buffer. Protein binding was detected using real-time PCR.
Positive control: Position 89340150-89340297 in chromosome 11 (has a validated c-Jun site).
Negative Control: Igr5 intron 3 (contains no c-Jun binding site).
Paraffin-embedded human breast carcinoma tissue stained for c-Jun with ab31419 at a 1/50 dilution in immunohistochemical analysis.
Left panel: Untreated.
Right panel: Pre-incubated with synthesized peptide.
ICC/IF image of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling c-Jun (green) with ab31419 at 1 µg/ml. The cells were fixed in 4% PFA (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab31419 at 1 µg/ml overnight at +4 °C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077 used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
All lanes : Anti-c-Jun antibody - ChIP Grade (ab31419) at 1/500 dilution
Lane 1 : Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells
Lane 2 : Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells with immunizing peptide
Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
c-Jun was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using ab31419 at a 1/500 dilution.
Lane 1: Control IgG IP in HEK-293T whole cell lysate.
Lane 2: ab31419 in HEK-293T whole cell lysate.
Lane 3: c-Jun in HEK-293T whole cell lysate 500 µg (Input).
For western blotting an HRP-conjugated swine anti-rabbit polyclonal was used as the secondary antibody.
Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab31419 (blue line) at a 1/300 dilution. The cells were prepared as follows: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight. Buffer was 0.1% sodium azide with FBS in phosphate buffered solution. Cells were fixed using paraformaldehyde and permeabilized using Triton X-100 and NP40. Cells were gated by isolating cell population from plot of SSC-A / FSA-A. The secondary antibody used was a FITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/100.
Anti-c-Jun antibody - ChIP Grade (ab31419) at 1/500 dilution +
Recombinant Human c-Jun protein (ab54318) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 30 seconds
This product has been referenced in:
- Lin B et al. c-Jun suppresses the expression of WNT inhibitory factor 1 through transcriptional regulation and interaction with DNA methyltransferase 1 in gallbladder cancer. Mol Med Rep 17:8180-8188 (2018). Read more (PubMed: 29693707) »
- Wang W et al. C/EBPß LIP and c-Jun synergize to regulate expression of the murine progesterone receptor. Mol Cell Endocrinol N/A:N/A (2018). Read more (PubMed: 29870755) »