• Product name
    Anti-c-Jun antibody [E254]
    See all c-Jun primary antibodies
  • Description
    Rabbit monoclonal [E254] to c-Jun
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human c-Jun aa 1-100 (N terminal). The exact sequence is proprietary.

  • Epitope
    ab32137 reacts with an epitope located in the N terminal region of c-Jun.
  • Positive control
    • WB: NIH 3T3, HeLa cell lysate ICC/IF HeLa IHC-P: Skin carcinoma IP: HEK-293, NIH3T3 cells.
  • General notes

    A trial size is available to purchase for this antibody.



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/250.
IP Use a concentration of 5 µg/ml.
ICC/IF 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
    • Sequence similarities
      Belongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
      Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
      Acetylated at Lys-271 by EP300.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • cJun antibody
      • Enhancer Binding Protein AP1 antibody
      • Jun Activation Domain Binding Protein antibody
      • JUN antibody
      • Jun oncogene antibody
      • JUN protein antibody
      • Jun proto oncogene antibody
      • JUN_HUMAN antibody
      • JUNC antibody
      • Oncogene JUN antibody
      • p39 antibody
      • Proto oncogene c jun antibody
      • Proto oncogene cJun antibody
      • Proto-oncogene c-jun antibody
      • Transcription Factor AP 1 antibody
      • Transcription factor AP-1 antibody
      • Transcription Factor AP1 antibody
      • V jun avian sarcoma virus 17 oncogene homolog antibody
      • V jun sarcoma virus 17 oncogene homolog (avian) antibody
      • V jun sarcoma virus 17 oncogene homolog antibody
      • V-jun avian sarcoma virus 17 oncogene homolog antibody
      • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all


    • Anti-c-Jun antibody [E254] (ab32137) at 1/50000 dilution + PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    • ab32137 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) (ab120618), by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.
      The cells were incubated at 37°C for 4h in media containing different concentrations of ab120618 (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32137 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • All lanes : Anti-c-Jun antibody [E254] (ab32137) at 1/2000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS Hot lysis method.
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates prepared in RIPA lysis method
      Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates prepared in 1% SDS Hot lysis method 15ug.

      Lysates/proteins at 15 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 39 kDa why is the actual band size different from the predicted?

      Exposure time: 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST

      For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Anti-c-Jun antibody [E254] (ab32137) at 1/2000 dilution + NIH 3T3 cell lysate

      Predicted band size: 36 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Immunohistochemical analysis of c-Jun expression in paraffin embedded skin carcinoma tissue sample, using 1/250 ab32137.

    • ab32137 (purified) at 1/500 dilution (2.29 µg/ml) immunoprecipitating c-Jun in HEK-293 whole cell lysate.
      Lane 2 (+): HEK-293(Human embronic kidney epithelial cell) whole cell lysate 10 µg
      Lane 3 (-): ab32137 & HEK-293 whole cell lysateRabbit monoclonal IgG (ab172730) instead of ab32137 in HEK-293 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM /TBST .

    • Chromatin was prepared from PC-12 (starve overnight) + NGF ( 50 ng/ml 2h) cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab32137 (blue), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5µg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    • c-Jun was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to c-Jun and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32137.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 45kDa; c-Jun
    • ICC/IF image of ab32137 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32137, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD



    This product has been referenced in:
    • Lakkappa N  et al. Soluble epoxide hydrolase inhibitor, APAU, protects dopaminergic neurons against rotenone induced neurotoxicity: Implications for Parkinson's disease. Neurotoxicology 70:135-145 (2019). Read more (PubMed: 30472438) »
    • Li Y  et al. Overexpression of Opa interacting protein 5 increases the progression of liver cancer via BMPR2/JUN/CHEK1/RAC1 dysregulation. Oncol Rep 41:2075-2088 (2019). Read more (PubMed: 30816485) »
    See all 43 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Western blot
    Human Cell lysate - whole cell (IM9 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin Gel, semi dry transfer on PVDF membrane)
    Loading amount
    25 µg
    IM9 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Mar 26 2019

    Immunocytochemistry/ Immunofluorescence
    Human Cell (AML-193)
    Yes - 0.1% Tritonx-100 10min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Sep 12 2017


    Thank you very much for your call this morning.
    As discussed, I would be happy to provide you with our testing discount to test one of our anti cFos or cJun antibodies in ChIP.
    This morning we discussed the antibodies anti cJun ab32137, anti cFos ab7963 and anti cFos ab129361.
    https://www.abcam.com/index.html?datasheet=32137 https://www.abcam.com/index.html?datasheet=7963
    The testing discount I can offer for each of these antiboides is a discount off a future purchase if you buy one of these antibodies now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know which antibodies you would like to test in ChIP. I will then send a discount code for each of the antibodies. This code must be issued before purchasing the antibodies, so please wait for my reply before ordering.
    2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).
    3. Test it in ChIP.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if an antibody turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up