Product nameAnti-c-Jun antibody [EP693Y]
See all c-Jun primary antibodies
DescriptionRabbit monoclonal [EP693Y] to c-Jun
SpecificityPBS only lot tested.
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human c-Jun aa 300-400 (C terminal). The exact sequence is proprietary.
- WB: PC-12 and NIH/3T3 whole cell lysate. HEK-293 cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab40766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/250.|
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
All lanes : Anti-c-Jun antibody [EP693Y] (ab40766) at 0.212 µg/ml
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1- 41 seconds
Lane 2 - 1 second
Anti-c-Jun antibody [EP693Y] (ab40766) at 1/5000 dilution + 10ug 293 cell lysate
Predicted band size: 39 kDa
Observed band size: 39 kDa
Tissue: Paraffin-embedded human colon carcinoma.
Staining: ab40766 at 1/100 dilution
Immunofluorescent staining of HeLa cells
ab40766 at 1/100 dilution
This product has been referenced in:
- Fernandes KA et al. Role of SARM1 and DR6 in retinal ganglion cell axonal and somal degeneration following axonal injury. Exp Eye Res 171:54-61 (2018). Read more (PubMed: 29526794) »
- Syc-Mazurek SB et al. JUN is important for ocular hypertension-induced retinal ganglion cell degeneration. Cell Death Dis 8:e2945 (2017). IHC ; Mouse . Read more (PubMed: 28726785) »