Anti-c-Jun (phospho S243) antibody (ab28846)
Key features and details
- Rabbit polyclonal to c-Jun (phospho S243)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Jun (phospho S243) antibody
See all c-Jun primary antibodies -
Description
Rabbit polyclonal to c-Jun (phospho S243) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human c-Jun (phospho S243). Immunogen is in the range of aa 210 - 259
Database link: P05412 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against nonphosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Corresponding non-phospho antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
Images
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All lanes : Anti-c-Jun (phospho S243) antibody (ab28846) at 1/500 dilution
Lane 1 : HeLa cell lysate (5-30ug) - untreated
Lane 2 : HeLa cell lysate (5-30ug) - treated with UV light
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
Ab28846, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma by Immunohistochemistry.
Left image: using c-Jun (phospho S243).
Right image: the same antibody preincubated with the corresponding synthesized phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab28846 has been referenced in 1 publication.
- Bienemann AS et al. Hsp70 suppresses apoptosis in sympathetic neurones by preventing the activation of c-Jun. J Neurochem 104:271-8 (2008). ICC/IF ; Rat . PubMed: 17971127