The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide.
The antibody against nonphosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
1/50 - 1/100.
Use at an assay dependent concentration.
Use at an assay dependent dilution.
Use at an assay dependent concentration. PubMed: 17971127
Use at an assay dependent dilution
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
Ab28846, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma by Immunohistochemistry. Left image: using c-Jun (phospho S243).
Right image: the same antibody preincubated with the corresponding synthesized phosphopeptide.