• Product name

    Anti-c-Jun (phospho S73) antibody
    See all c-Jun primary antibodies
  • Description

    Rabbit polyclonal to c-Jun (phospho S73)
  • Host species

  • Specificity

    ab30620 detects endogenous levels of c-Jun only when phosphorylated at Serine 73.
  • Tested applications

    Suitable for: ICC/IF, IHC - Wholemount, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.

  • Positive control

    • Breast carcinoma. extracts from Hela cells treated with UV.



Our Abpromise guarantee covers the use of ab30620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC - Wholemount 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • Function

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similarities

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational

    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP-1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all


  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.

  • All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution

    Lane 1 : extracts from untreated Hela cells
    Lane 2 : extracts from UV treated Hela cells

    Predicted band size: 36 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

  • ab30620 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab30620 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab30620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Zheng XM  et al. MicroRNA-30e inhibits adhesion, migration, invasion and cell cycle progression of prostate cancer cells via inhibition of the activation of the MAPK signaling pathway by downregulating CHRM3. Int J Oncol 54:443-454 (2019). Read more (PubMed: 30483762) »
  • Tang Q  et al. miR-223/Hsp70/JNK/JUN/miR-223 feedback loop modulates the chemoresistance of osteosarcoma to cisplatin. Biochem Biophys Res Commun 497:827-834 (2018). WB . Read more (PubMed: 29432736) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab30620 has never been tested in frozen sections (IHC-Fr). All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

I would suggest it is very likely to work, at it has been used successfully in IHC-P, ICC-IF and wholemount. However, I am sorry we are not able to provide a guarantee for this without testing.

If you try this in IHC-Fr, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


I hope this information is helpful. Please do not hesitate to contact me if you have any further questions.

Read More
IHC - Wholemount
Zebrafish Embryo (all embryo)
all embryo

Abcam user community

Verified customer

Submitted Mar 14 2012

Western blot
Human Tissue lysate - whole (Brain tumor)
Loading amount
10 µg
Brain tumor
Lysis buffer (urea/thiourea)
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 05 2010

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