Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-c-Jun (phospho T91 + T93) antibody [C-J 4C4]
See all c-Jun primary antibodies -
DescriptionMouse monoclonal [C-J 4C4] to c-Jun (phospho T91 + T93)
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
c-Jun protein phosphorylated at T91 and T93.
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A/G purified
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ClonalityMonoclonal
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Clone numberC-J 4C4
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab13671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
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Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3725 Human
- Entrez Gene: 24516 Rat
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P17325 Rat
- Unigene: 696684 Human
- Unigene: 93714 Rat
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)](https://www.abcam.com/ps/products/13/ab13671/Images/c-Jun-Primary-antibodies-ab13671-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)ICC/IF image of ab13617 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)](https://www.abcam.com/ps/products/13/ab13671/Images/c-Jun-Primary-antibodies-ab13671-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)IHC image of c-Jun (phospho T91 + T93) staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- Hu G et al. MicroRNA-145 attenuates TNF-a-driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4. Cell Death Dis 8:e3140 (2017). IHC-P ; Human . Read more (PubMed: 29072705) »
- Kudo K et al. Inhibition of Gli1 results in altered c-Jun activation, inhibition of cisplatin-induced upregulation of ERCC1, XPD and XRCC1, and inhibition of platinum-DNA adduct repair. Oncogene 31:4718-24 (2012). Human . Read more (PubMed: 22266871) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"