• Product name
    Anti-c-Kit antibody [2B8] (Biotin)
    See all c-Kit primary antibodies
  • Description
    Rat monoclonal [2B8] to c-Kit (Biotin)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, Functional Studies, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.


Our Abpromise guarantee covers the use of ab25022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


Functional Studies Use at an assay dependent concentration. Can be used for cell homing studies, blockade of hematopoiesis and disruption fo melanocyte development.
ICC/IF Use at an assay dependent concentration.


  • Function
    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
  • Tissue specificity
    Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
  • Involvement in disease
    Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
  • Cellular localization
    Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Information by UniProt
  • Database links
  • Alternative names
    • C Kit antibody
    • c-Kit antibody
    • c-Kit Ligand antibody
    • CD117 antibody
    • Kit antibody
    • Kit Ligand antibody
    • KIT oncogene antibody
    • KIT proto oncogene receptor tyrosine kinase antibody
    • KIT_HUMAN antibody
    • Mast cell growth factor receptor antibody
    • Mast/stem cell growth factor receptor Kit antibody
    • MGF antibody
    • p145 c-kit antibody
    • PBT antibody
    • Piebald trait protein antibody
    • Proto oncogene c Kit antibody
    • Proto oncogene tyrosine protein kinase Kit antibody
    • Proto-oncogene c-Kit antibody
    • SCF Receptor antibody
    • SCFR antibody
    • soluble KIT variant 1 antibody
    • Steel Factor Receptor antibody
    • Stem cell factor receptor antibody
    • tyrosine protein kinase Kit antibody
    • Tyrosine-protein kinase Kit antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
    • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
    see all


  • ab25022 staining c-Kit (red) in murine testis tissue sections by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/100 in 2% BSA + 0.005% Triton X-100 in PBS) for 16 hours at 4°C. Avidin-FITC was used for detection.

    Nuclei were stained with DAPI (blue).

    See Abreview

  • Flow Cytometry analysis of C57BL/6 mouse bone marrow cells labeling c-Kit with ab25022 at 0.3 μg/106 cells dilution. Rat Anti-Mouse CD45R-PE followed by Streptavidin-FITC was used as the secondary antibody. 

  • Immunostaining of mouse embryonic stem cells using c-Kit (Biotin) ab25022. The antibody bound to the cells was detected by treatment with Cy3-conjugated streptavidin (25 µg/ml). The image shows a fragment of an adherent colony of mouse embryonic stem cell-derived hepatocyte precursor cells stained with the ab25022 antibody and subsequently treated with Cy3-conjugated streptavidin. Cell nuclei were stained with DAPI. The Cy3 fluorescence pattern suggests the predominant expression of c-Kit in actively proliferating cells in the middle of colonies. Please see abreview for ICC/IF protocol details.

    See Abreview


This product has been referenced in:
  • Parada-Kusz M  et al. Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies. Dis Model Mech 11:N/A (2018). Read more (PubMed: 30266803) »
  • Trias E  et al. Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS. JCI Insight 2:N/A (2017). Read more (PubMed: 29046475) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Mouse Tissue sections (Left Ventricle)
Left Ventricle

Abcam user community

Verified customer

Submitted Aug 20 2014


Earlier we spoke of the non-specific staining and lack of expected, positive staining in your IHC sections. We discussed attempting antigen retrieval at 95-100C rather than 80C and trying some alternative buffers (Ex. Tris-EDTA) and potentially an enzymatic antigen retrieval at 37C using proteinase K or pepsin.

Read More


Thank you for contacting us.

I am sorry to hear thatone of the antibodies is not working as expected.

According to the image you had sent, it seems that the c-kit antibody (ab25022) is not working correctly. However the vimentin antibody ab128507 seems to stain the cytoplasm correctly. I am not aware that vimentin should only be present in fibroblasts, it is highly expressed there, but can also be present in other cell types.
What part oftissue (fibroblasts, cardiomyocytes) does the image you sent represent?

Please let me know these details so that I can assist you better with troubleshooting.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!

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Thank you for contacting us.

Although we have not determined the affinity of this antibody against the rat orthologue, we are pleased to let you know that we guarantee this c-Kit antibody ab25022 to work with rat samples. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

For a biotin-conjugated antibody like ab25022 in general a secondary anti-biotin antibody such as ab1227 could be used or a streptavidin-conjugate such as ab7403. However, as I am not experienced with MACS cell separation I am not quite sure which products are most suitable for you. I would recommend enquiring with the manufacturer of your MACS instruments about the choice of secondaries and magnetic microbeads.

I am sorry I could not be of more assistance on this occasion, but I hope this information is nevertheless helpful for you. Please do not hesitate to contact us again with any other questions.

Read More


Thank you for your enquiry. I have looked through our stock of c-Kit antibodies and unfortunately have not found one where references we know of support its use to block receptor function. I am sorry I could not be of more help in this instance. Please feel free to contact me if you have any further questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Rat Cell (Cardiac progenitor cell)
Cardiac progenitor cell
Cell harvesting/tissue preparation method: Cardiomycyte isolation
Sample buffer: Kreb's
Gating Strategy
Cardiac Progenitor Cells

Abcam user community

Verified customer

Submitted Oct 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (testis)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Embryonic stem cell-derived hepatocyte precursors)
Embryonic stem cell-derived hepatocyte precursors
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Irina Drobinskaya

Verified customer

Submitted Feb 17 2009


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