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  1. Link

    c-kit-antibody-yr145-ab32363.pdf

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Immunology Cell Type Markers CD Cytokines
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-c-Kit antibody [YR145] (ab32363)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (42)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
  • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
  • OI-RD Scanning - Anti-c-Kit antibody [YR145] (ab32363)
  • Anti-c-Kit antibody [YR145] (ab32363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YR145] to c-Kit
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human, Common marmoset

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Overview

  • Product name

    Anti-c-Kit antibody [YR145]
    See all c-Kit primary antibodies
  • Description

    Rabbit monoclonal [YR145] to c-Kit
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to c-Kit. It does not crossreact with other CSF1/PDGF receptor family member.
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human, Common marmoset
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, fetal lung and seminoma tissue lysate. IHC-P: Human lung adenocarcinoma and seminoma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 5.90 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    YR145
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-c-Kit antibody [YR145] - BSA and Azide free (ab216450)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human c-Kit protein (Fc Chimera) (ab88349)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (4)
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB (1)
1/1000. Predicted molecular weight: 110 kDa.
Notes
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/1000. Predicted molecular weight: 110 kDa.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
  • Tissue specificity

    Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
  • Involvement in disease

    Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
  • Cellular localization

    Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Target information above from: UniProt accession P10721 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3815 Human
    • Omim: 164920 Human
    • SwissProt: P10721 Human
    • Unigene: 479754 Human
    • Alternative names

      • C Kit antibody
      • c-Kit antibody
      • c-Kit Ligand antibody
      • CD117 antibody
      • Kit antibody
      • Kit Ligand antibody
      • KIT oncogene antibody
      • KIT proto oncogene receptor tyrosine kinase antibody
      • KIT_HUMAN antibody
      • Mast cell growth factor receptor antibody
      • Mast/stem cell growth factor receptor Kit antibody
      • MGF antibody
      • p145 c-kit antibody
      • PBT antibody
      • Piebald trait protein antibody
      • Proto oncogene c Kit antibody
      • Proto oncogene tyrosine protein kinase Kit antibody
      • Proto-oncogene c-Kit antibody
      • SCF Receptor antibody
      • SCFR antibody
      • soluble KIT variant 1 antibody
      • Steel Factor Receptor antibody
      • Stem cell factor receptor antibody
      • tyrosine protein kinase Kit antibody
      • Tyrosine-protein kinase Kit antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
      • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling c-Kit with unpurified ab32363.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      All lanes : Anti-c-Kit antibody [YR145] (ab32363) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : KIT knockout HAP1 whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 110 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab32363 observed at 109 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab32363 was shown to recognize 0 in wild-type HAP1 cells as signal was lost at the expected MW in KIT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KIT knockout samples were subjected to SDS-PAGE. Ab32363 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling c-Kit with purified ab32363 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      Anti-c-Kit antibody [YR145] (ab32363) at 1/5000 dilution (purified) + Human seminoma tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size: 110 kDa
      Observed band size: 120-145 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      Western blot - Anti-c-Kit antibody [YR145] (ab32363)
      Anti-c-Kit antibody [YR145] (ab32363) at 1/1000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size: 110 kDa
      Observed band size: 120-145 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling c-Kit with unpurified ab32363.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling c-Kit with unpurified ab32363.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling c-Kit with unpurified ab32363.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Kit antibody [YR145] (ab32363)This image is courtesy of an abreview from Zachary Yu-Ching Lin.

      IHC-P image of c-Kit (unpurified ab32363) staining on adult marmoset testis. The sections were fixed in formaldehyde and underwent heat mediated antigen retrieval, using Dako antigen retrieval solution. The sections were then blocked in 5% milk for 30 mins at 25°C. The primary antibody was added at a 1/100 dilution, and then incubated for 18 hours at 4°C. Dapi was used to stain the nuclei (blue).

      See Abreview

    • OI-RD Scanning - Anti-c-Kit antibody [YR145] (ab32363)
      OI-RD Scanning - Anti-c-Kit antibody [YR145] (ab32363)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-c-Kit antibody [YR145] (ab32363)
      Anti-c-Kit antibody [YR145] (ab32363)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (42)

    Publishing research using ab32363? Please let us know so that we can cite the reference in this datasheet.

    ab32363 has been referenced in 42 publications.

    • Liu J  et al. Exosomes from tamoxifen-resistant breast cancer cells transmit drug resistance partly by delivering miR-9-5p. Cancer Cell Int 21:55 (2021). PubMed: 33451320
    • Yuan H  et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
    • Tang F  et al. Patient-Derived Tumor Xenografts Plus Ex Vivo Models Enable Drug Validation for Tenosynovial Giant Cell Tumors. Ann Surg Oncol N/A:N/A (2021). PubMed: 33748895
    • Wang M  et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
    • Li Q  et al. Identification of the EMT-Related Genes Signature for Predicting Occurrence and Progression in Thyroid Cancer. Onco Targets Ther 14:3119-3131 (2021). PubMed: 34012269
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Melanoma cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 09 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Melanomcytes)
    Permeabilization
    No
    Specification
    Melanomcytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 17 2021

    Immunocytochemistry abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Melanoma cells)
    Permeabilization
    No
    Specification
    Melanoma cells
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 03 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hair follicle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Hair follicle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 05 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-c-Kit antibody [YR145]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Baboon Tissue sections (fetal liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH, 6.0
    Permeabilization
    No
    Specification
    fetal liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Marcia Burch

    Verified customer

    Submitted Mar 04 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-c-Kit antibody [YR145]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Small Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate (pH 6)
    Permeabilization
    No
    Specification
    Small Intestine
    Blocking step
    Dako Serum-free Protein Block lot 10141888 ref x0909 as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 15 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-c-Kit antibody [YR145]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (Atria)
    Permeabilization
    Yes - 0.3% TX-100,10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Specification
    Atria
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Juan Estrada

    Verified customer

    Submitted Apr 05 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-c-Kit antibody [YR145]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Mar 27 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-c-Kit antibody [YR145]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (adult testis)
    Specification
    adult testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    煜欽 林

    Verified customer

    Submitted Feb 06 2013

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