Product nameAnti-c-Maf antibody [BLR045F]
See all c-Maf primary antibodies
DescriptionRabbit monoclonal [BLR045F] to c-Maf
Tested applicationsSuitable for: ICC, IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human c-Maf aa 125-175. The exact sequence is proprietary. NP_005351.2 and Gene ID 4094.
Database link: O75444
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 6µl/mg lysate.
FunctionActs as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant reponse element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
Tissue specificityExpressed in endothelial cells.
Involvement in diseaseNote=A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Translocation t(14;16)(q32.3;q23) with an IgH locus.
Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM) [MIM:610202]. A form of cataract with nuclear or cortical pulverulent opacities. Pulverulent cataracts are characterized by a dust-like, 'pulverised' appearance of the opacities which can be found in any part of the lens. The phenotype shows significant intra- and interfamilial variation, both in the distribution of the cataract and the degree of opacification. Some patients with cataract pulverulent juvenile-onset can present microcornea and bilateral iris colobomas in addition to cataract.
Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4) [MIM:610202]. A cerulean form of congenital cataract. Cerulean cataracts are characterized by peripheral bluish and white opacifications organized in concentric layers with occasional central lesions arranged radially. The opacities are observed in the superficial layers of the fetal nucleus as well as the adult nucleus of the lens. Involvement is usually bilateral. Visual acuity is only mildly reduced in childhood. In adulthood, the opacifications may progress, making lens extraction necessary. Histologically the lesions are described as fusiform cavities between lens fibers which contain a deeply staining granular material. Although the lesions may take on various colors, a dull blue is the most common appearance and is responsible for the designation cerulean cataract.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
modificationsUbiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids.
Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.
- Information by UniProt
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c-Maf was immunoprecipitated from 1.0 mg RPMI 8226 whole cell lysate with ab243901 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243901 at 1/1000 dilution.
Lane 1: ab243901 IP in RPMI 8226 whole cell lysate.
Lane 2: Control IgG in RPMI 8226 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human and mouse cMaf by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or cMaf using ab243901. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Rabbit anti-Myc.
Western blot analysis using ab243901 at 1/1000 dilution.
Lane 1: HeLa cell whole cell lysate (50 µg).
Lane 2: HEK-293T cell whole cell lysate (50 µg).
Lane 3: A549 whole cell lysate (50 µg).
Lane 4: RPMI 8226 whole cell lysate (50 µg).
Lane 5: HepG2 whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: 786-O whole cell lysate (50 µg).
Lane 8: MCF7 whole cell lysate (50 µg).
Lane 9: K562 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Formalin-fixed, paraffin-embedded RPMI 8226 cells c-Mafa using ab243901 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
ab243901 has not yet been referenced specifically in any publications.