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  1. Link

    c-maf-antibody-blr045f-bsa-free-ab243901.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Recombinant

Recombinant Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
  • Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR045F] to c-Maf - BSA free
  • Suitable for: ICC, IHC-P, IP, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-c-Maf antibody [BLR045F] - BSA free
    See all c-Maf primary antibodies
  • Description

    Rabbit monoclonal [BLR045F] to c-Maf - BSA free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human c-Maf aa 125-175. The exact sequence is proprietary. NP_005351.2 and Gene ID 4094.
    Database link: O75444

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituent: 99% Borate buffered saline
  • Concentration information loading...
  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BLR045F
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Immunology
    • Immune System Diseases
    • Allergens
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Recombinant Protein

    • Recombinant Human c-Maf protein (ab114469)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/100 - 1/500.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

Use 6µl/mg lysate.

WB
1/1000.
Notes
ICC
1/100 - 1/500.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

Use 6µl/mg lysate.

WB
1/1000.

Target

  • Function

    Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant reponse element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
  • Tissue specificity

    Expressed in endothelial cells.
  • Involvement in disease

    Note=A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Translocation t(14;16)(q32.3;q23) with an IgH locus.
    Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM) [MIM:610202]. A form of cataract with nuclear or cortical pulverulent opacities. Pulverulent cataracts are characterized by a dust-like, 'pulverised' appearance of the opacities which can be found in any part of the lens. The phenotype shows significant intra- and interfamilial variation, both in the distribution of the cataract and the degree of opacification. Some patients with cataract pulverulent juvenile-onset can present microcornea and bilateral iris colobomas in addition to cataract.
    Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4) [MIM:610202]. A cerulean form of congenital cataract. Cerulean cataracts are characterized by peripheral bluish and white opacifications organized in concentric layers with occasional central lesions arranged radially. The opacities are observed in the superficial layers of the fetal nucleus as well as the adult nucleus of the lens. Involvement is usually bilateral. Visual acuity is only mildly reduced in childhood. In adulthood, the opacifications may progress, making lens extraction necessary. Histologically the lesions are described as fusiform cavities between lens fibers which contain a deeply staining granular material. Although the lesions may take on various colors, a dull blue is the most common appearance and is responsible for the designation cerulean cataract.
  • Sequence similarities

    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications

    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids.
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O75444 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4094 Human
    • Entrez Gene: 17132 Mouse
    • Omim: 177075 Human
    • SwissProt: O75444 Human
    • SwissProt: P54843 Mouse
    • Unigene: 134859 Human
    • Unigene: 275549 Mouse
    • Unigene: 410395 Mouse
    • Unigene: 439772 Mouse
    see all
  • Alternative names

    • AS42 oncogene homolog antibody
    • Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene antibody
    • Avian musculoaponeurotic fibrosarcoma (v maf) antibody
    • c maf proto oncogene antibody
    • cMaf antibody
    • maf antibody
    • MAF_HUMAN antibody
    • MAF2 antibody
    • MGC71685 antibody
    • Proto oncogene c Maf antibody
    • Proto-oncogene c-maf antibody
    • Transcription factor Maf antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) antibody
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog antibody
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog antibody
    see all

Images

  • Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Immunoprecipitation - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    c-Maf was immunoprecipitated from 1.0 mg RPMI 8226 whole cell lysate with ab243901 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243901 at 1/1000 dilution.

    Lane 1: ab243901 IP in RPMI 8226 whole cell lysate.

    Lane 2: Control IgG in RPMI 8226 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

    Detection of human and mouse cMaf by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or cMaf using ab243901. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Rabbit anti-Myc.

  • Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Western blot - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

    Western blot analysis using ab243901 at 1/1000 dilution.

    Lane 1: HeLa cell whole cell lysate (50 µg).

    Lane 2: HEK-293T cell whole cell lysate (50 µg).

    Lane 3: A549 whole cell lysate (50 µg).

    Lane 4: RPMI 8226 whole cell lysate (50 µg).

    Lane 5: HepG2 whole cell lysate (50 µg).

    Lane 6: Jurkat whole cell lysate (50 µg).

    Lane 7: 786-O whole cell lysate (50 µg).

    Lane 8: MCF7 whole cell lysate (50 µg).

    Lane 9: K562 whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.

  • Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Immunocytochemistry - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Formalin-fixed, paraffin-embedded RPMI 8226 cells c-Mafa using ab243901 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Maf using ab243901 at 1/125 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
  • Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)
    Anti-c-Maf antibody [BLR045F] - BSA free (ab243901)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab243901? Please let us know so that we can cite the reference in this datasheet.

ab243901 has been referenced in 1 publication.

  • Granai M  et al. Immune landscape in Burkitt lymphoma reveals M2-macrophage polarization and correlation between PD-L1 expression and non-canonical EBV latency program. Infect Agent Cancer 15:28 (2020). PubMed: 32391073

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