Anti-c-Myb antibody (ab117635)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-c-Myb antibody
See all c-Myb primary antibodies -
Description
Rabbit polyclonal to c-Myb -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
KLH-conjugated synthetic peptide derived from the N terminal region of Human c-Myb.
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Positive control
- Human breast or tonisl tissue. HUVEC cell lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 48% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Domain
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 606320 Human
- Unigene: 52109 Mouse
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Alternative names
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
Images
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All lanes : Anti-c-Myb antibody (ab117635) at 1/500 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate with synthetic peptide
Predicted band size: 72 kDa -
Immunofluorescence analysis of HeLa cells, using 1/100 ab117635.
The picture on the right is treated with the synthesized peptide. -
Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml.
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Human Tonsil, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab117635 has been referenced in 7 publications.
- Liu Q et al. Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter. J Cancer 12:1990-1999 (2021). PubMed: 33753997
- Simeoni F et al. Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Cell Rep 36:109725 (2021). PubMed: 34551306
- Kodric K et al. Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis. Exp Mol Med 51:1-16 (2019). PubMed: 31409771
- Felipe-Abrio B et al. Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer. Cancers (Basel) 11:N/A (2019). PubMed: 30781655
- Xie R et al. c-Myb and its Effector COX-2 as an Indicator Associated with Prognosis and Therapeutic Outcome in Colorectal Cancer. J Cancer 10:1601-1610 (2019). PubMed: 31205515
- Ringleb J et al. Apoptotic Cancer Cells Suppress 5-Lipoxygenase in Tumor-Associated Macrophages. J Immunol 200:857-868 (2018). PubMed: 29229677
- Tosello V et al. A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia. Cells 7:N/A (2018). PubMed: 30304769