Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-c-Myb antibody
See all c-Myb primary antibodies
DescriptionRabbit polyclonal to c-Myb
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- Human breast carcinoma tissue; MOLT4 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177510 was affinity purified using an epitope specific to c-Myb immobilized on solid support.
Our Abpromise guarantee covers the use of ab177510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
Detection of Human c-Myb by Western Blot of Immunoprecipitate.
Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT4 cells.
Lane 2: Control IgG.
ab177510 used for IP at 6 μg/mg lysate.
ab177510 was used at 1 μg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling c-Myb with ab177510 at 1µg/ml. Detection:DAB.
ab177510 has been referenced in 1 publication.
- Godfrey L et al. DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nat Commun 10:2803 (2019). PubMed: 31243293