Overview

  • Product name

  • Description

    Rabbit polyclonal to c-Myb
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human c-Myb aa 525-575. The exact sequence is proprietary. (NP_005366.2).
    Database link: P10242

  • Positive control

    • WB: MOLT-4 and Jurkat whole cell lysate (ab7899). IP: MOLT-4 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226251 was affinity purified using an epitope specific to c-Myb immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • Sequence similarities

    Contains 3 HTH myb-type DNA-binding domains.
  • Domain

    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-c-Myb antibody (ab226251) at 0.1 µg/ml

    Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
    Lane 2 : MOLT-4 whole cell lysate at 15 µg
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 10 seconds
  • c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226251 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226251 at 0.4 µg/ml.

    Lane 1: ab226251 IP in MOLT-4 whole cell lysate.

    Lane 2: Control IgG IP in MOLT-4 whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226251.
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