Overview

  • Product name

  • Description

    Rabbit polyclonal to c-Myb
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human c-Myb aa 1-50. The exact sequence is proprietary. (NP_005366.2).
    Database link: P10242

  • Positive control

    • IP: MOLT-4 whole cell lysate. IHC-P: Human salivary gland and breast carcinoma tissues; Mouse renal cell carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226470 was affinity purified using an epitope specific to c-Myb immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226470 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-c-Myb antibody at 1 µg/ml.

      Lane 1: ab226470 IP in MOLT-4 whole cell lysate.

      Lane 2: Control IgG IP in MOLT-4 whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c-Myb using ab226470 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded human salivary gland tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    References

    ab226470 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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