Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-c-Myb antibody
See all c-Myb primary antibodies
DescriptionRabbit polyclonal to c-Myb
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human c-Myb aa 1-50. The exact sequence is proprietary. (NP_005366.2).
Database link: P10242
- IP: MOLT-4 whole cell lysate. IHC-P: Human salivary gland and breast carcinoma tissues; Mouse renal cell carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226470 was affinity purified using an epitope specific to c-Myb immobilized on solid support.
Our Abpromise guarantee covers the use of ab226470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226470 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-c-Myb antibody at 1 µg/ml.
Lane 1: ab226470 IP in MOLT-4 whole cell lysate.
Lane 2: Control IgG IP in MOLT-4 whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c-Myb using ab226470 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Formalin-fixed, paraffin-embedded human salivary gland tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226470 has been referenced in 1 publication.
- Kodric K et al. Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis. Exp Mol Med 51:1-16 (2019). PubMed: 31409771