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    c-myb-antibody-ab62824.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes
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Anti-c-Myb antibody (ab62824)

  • Datasheet
Reviews (1)Q&A (1)References (3)

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Western blot - Anti-c-Myb antibody (ab62824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)

Key features and details

  • Goat polyclonal to c-Myb
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-c-Myb antibody
    See all c-Myb primary antibodies
  • Description

    Goat polyclonal to c-Myb
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-QRHYNDEDPEKEKR

    , corresponding to internal sequence amino acids 281-294 of Human c-Myb.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Human Spleen lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

Our Abpromise guarantee covers the use of ab62824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Function

    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • Sequence similarities

    Contains 3 HTH myb-type DNA-binding domains.
  • Domain

    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P10242 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 463016 Chimpanzee
    • Entrez Gene: 317776 Cow
    • Entrez Gene: 483995 Dog
    • Entrez Gene: 4602 Human
    • Entrez Gene: 17863 Mouse
    • Entrez Gene: 498982 Rat
    • Omim: 189990 Human
    • SwissProt: P46200 Cow
    • SwissProt: P10242 Human
    • SwissProt: P06876 Mouse
    • Unigene: 606320 Human
    • Unigene: 52109 Mouse
    see all
  • Alternative names

    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Images

  • Western blot - Anti-c-Myb antibody (ab62824)
    Western blot - Anti-c-Myb antibody (ab62824)
    Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg

    Predicted band size: 72 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Detected by chemiluminescence
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    ab62824 at 4µg/ml staining c-Myb in Human Kidney tissue. Steamed antigen retrieval with EDTA buffer pH 9 was performed, and the sample was stained with HRP.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    ab62824 at 4µg/ml staining c-Myb in Human Breast tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed, and the sample was stained with HRP.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab62824? Please let us know so that we can cite the reference in this datasheet.

    ab62824 has been referenced in 3 publications.

    • Wang F  et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
    • Yi T  et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . PubMed: 26018492
    • Zheng J  et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). PubMed: 23604143

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I have used ab62824 IHC-P, IHC-Fr and ICC and there is no staining. Have used pH 6 retrieval buffer which has worked for other reviewers; I have tried fluorogeneic and chromogeneic detection systems and still no signal; i am using tumor samples that have been shown to express c-Myb based on other assays.

    Read More

    Abcam community

    Verified customer

    Asked on May 25 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab117635.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on May 25 2012

    Western blot abreview for Anti-c-Myb antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse haematopoietic progenitor cell line)
    Loading amount
    40 µg
    Specification
    mouse haematopoietic progenitor cell line
    Gel Running Conditions
    Reduced Denaturing (7.5% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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