- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-c-Myb antibody
See all c-Myb primary antibodies -
DescriptionGoat polyclonal to c-Myb
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
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Immunogen
Synthetic peptide:
C-QRHYNDEDPEKEKR
, corresponding to internal sequence amino acids 281-294 of Human c-Myb. -
Positive control
- Human Spleen lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab62824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3 - 6 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). |
Target
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FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
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Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
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DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
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Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 463016 Chimpanzee
- Entrez Gene: 317776 Cow
- Entrez Gene: 483995 Dog
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Entrez Gene: 498982 Rat
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
Alternative names
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
Images
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Western blot - Anti-c-Myb antibody (ab62824)Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Predicted band size: 72 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Detected by chemiluminescence -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)ab62824 at 4µg/ml staining c-Myb in Human Kidney tissue. Steamed antigen retrieval with EDTA buffer pH 9 was performed, and the sample was stained with HRP. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)ab62824 at 4µg/ml staining c-Myb in Human Breast tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed, and the sample was stained with HRP.
References
This product has been referenced in:
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . Read more (PubMed: 26018492) »
- Zheng J et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). Read more (PubMed: 23604143) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"