Anti-c-Myb antibody (ab62824)
Key features and details
- Goat polyclonal to c-Myb
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-c-Myb antibody
See all c-Myb primary antibodies -
Description
Goat polyclonal to c-Myb -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
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Immunogen
Synthetic peptide:
C-QRHYNDEDPEKEKR
, corresponding to internal sequence amino acids 281-294 of Human c-Myb. -
Positive control
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Human Spleen lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab62824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3 - 6 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Domain
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 463016 Chimpanzee
- Entrez Gene: 317776 Cow
- Entrez Gene: 483995 Dog
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Entrez Gene: 498982 Rat
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
Alternative names
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
Images
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Western blot - Anti-c-Myb antibody (ab62824)Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Detected by chemiluminescence -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)ab62824 at 4µg/ml staining c-Myb in Human Kidney tissue. Steamed antigen retrieval with EDTA buffer pH 9 was performed, and the sample was stained with HRP. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)ab62824 at 4µg/ml staining c-Myb in Human Breast tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed, and the sample was stained with HRP.
Protocols
Datasheets and documents
References (3)
ab62824 has been referenced in 3 publications.
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . PubMed: 26018492
- Zheng J et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). PubMed: 23604143
Customer reviews and Q&As
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