Anti-c-Myb antibody (ab62824)

Reviews (1)Q&A (1)References (3)

Overview

  • Product name

    Anti-c-Myb antibody
    See all c-Myb primary antibodies

  • Description

    Goat polyclonal to c-Myb

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish

  • Immunogen

    Synthetic peptide:

    C-QRHYNDEDPEKEKR

    , corresponding to internal sequence amino acids 281-294 of Human c-Myb.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Human Spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab62824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

Target

  • Function

    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

  • Sequence similarities

    Contains 3 HTH myb-type DNA-binding domains.

  • Domain

    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Images

  • Western blot - Anti-c-Myb antibody (ab62824)
    Western blot - Anti-c-Myb antibody (ab62824)
    Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg

    Predicted band size: 72 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Detected by chemiluminescence
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    ab62824 at 4µg/ml staining c-Myb in Human Kidney tissue. Steamed antigen retrieval with EDTA buffer pH 9 was performed, and the sample was stained with HRP.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)
    ab62824 at 4µg/ml staining c-Myb in Human Breast tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed, and the sample was stained with HRP.

References

This product has been referenced in:

  • Wang F  et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). Read more (PubMed: 28244490) »
  • Yi T  et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . Read more (PubMed: 26018492) »
See all 3 Publications for this product

Publishing research using ab62824? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I have used ab62824 IHC-P, IHC-Fr and ICC and there is no staining. Have used pH 6 retrieval buffer which has worked for other reviewers; I have tried fluorogeneic and chromogeneic detection systems and still no signal; i am using tumor samples that have been shown to express c-Myb based on other assays.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab117635.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Western blot abreview for Anti-c-Myb antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse haematopoietic progenitor cell line)
Loading amount
40 µg
Specification
mouse haematopoietic progenitor cell line
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

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Verified customer

Submitted Jun 06 2011

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