Key features and details
- Rat monoclonal [ANA236B] to c-Myb
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-c-Myb antibody [ANA236B]
See all c-Myb primary antibodies
DescriptionRat monoclonal [ANA236B] to c-Myb
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: IHC-Fr or IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM
Database link: P10242
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab169111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Predicted molecular weight: 72 kDa.|
|ICC||Use at an assay dependent concentration.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.
Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human lymphoblastic lymphoma tissue labeling c-Myb with ab169111 at 1/80 dilution.
Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling c-Myb with ab169111 at 1/80 dilution.
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling c-Myb with ab169111 at 1/80 dilution.
All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution
Lane 1 : DAUDI cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Raji cell lysate
Lane 4 : KARPAS299 cell lysate
Lane 5 : HUT78 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MT2 cell lysate
Lane 8 : SUP-T1 cell lysate
Lane 9 : YT cell lysate
Lysates/proteins at 150 µg per lane.
Predicted band size: 72 kDa
Immunocytochemical analysis of Hek-V5-c-Myb transfected cells cytospins (right image) labeling c-Myb using ab169111. ab169111 does not detect other Myb proteins in Hek-V5-a-Myb transfected cells cytospins (left image) or in Hek-V5-b-Myb transfected cells cytospins (middle image).
ab169111 has not yet been referenced specifically in any publications.