Overview

  • Product name
    Anti-c-Myb antibody [ANA236B]
    See all c-Myb primary antibodies
  • Description
    Rat monoclonal [ANA236B] to c-Myb
  • Host species
    Rat
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
    Sequence:

    FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM


    Database link: P10242

  • Positive control
    • Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    ANA236B
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 72 kDa.
IHC-P 1/80.
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    • Function
      Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
    • Sequence similarities
      Contains 3 HTH myb-type DNA-binding domains.
    • Domain
      Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C myb antibody
      • c myb protein (140 AA) antibody
      • c-myb protein (140 AA) antibody
      • c-myb_CDS antibody
      • c-myb10A_CDS antibody
      • c-myb13A_CDS antibody
      • c-myb14A_CDS antibody
      • c-myb8B_CDS antibody
      • Cmyb antibody
      • efg antibody
      • Myb antibody
      • MYB proto-oncogene transcription factor antibody
      • MYB_HUMAN antibody
      • ONCOGENE AMV antibody
      • Oncogene MYB antibody
      • Proto oncogene c Myb antibody
      • Proto-oncogene c-Myb antibody
      • Transcriptional activator Myb antibody
      • v myb avian myeloblastosis viral oncogene homolog antibody
      • v-myb avian myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.

      Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human lymphoblastic lymphoma tissue labeling c-Myb with ab169111 at 1/80 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling c-Myb with ab169111 at 1/80 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling c-Myb with ab169111 at 1/80 dilution.

    • All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution

      Lane 1 : DAUDI cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : KARPAS299 cell lysate
      Lane 5 : HUT78 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : MT2 cell lysate
      Lane 8 : SUP-T1 cell lysate
      Lane 9 : YT cell lysate

      Lysates/proteins at 150 µg per lane.

      Predicted band size: 72 kDa

    • Immunocytochemical analysis of Hek-V5-c-Myb transfected cells cytospins (right image) labeling c-Myb using ab169111. ab169111 does not detect other Myb proteins in Hek-V5-a-Myb transfected cells cytospins (left image) or in Hek-V5-b-Myb transfected cells cytospins (middle image).

    References

    ab169111 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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