Product nameAnti-c-Myb antibody [ANA236B]
See all c-Myb primary antibodies
DescriptionRat monoclonal [ANA236B] to c-Myb
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: IHC-Fr or IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM
Database link: P10242
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab169111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Predicted molecular weight: 72 kDa.|
|ICC||Use at an assay dependent concentration.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.
Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human lymphoblastic lymphoma tissue labeling c-Myb with ab169111 at 1/80 dilution.
Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling c-Myb with ab169111 at 1/80 dilution.
Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling c-Myb with ab169111 at 1/80 dilution.
All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution
Lane 1 : DAUDI cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Raji cell lysate
Lane 4 : KARPAS299 cell lysate
Lane 5 : HUT78 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MT2 cell lysate
Lane 8 : SUP-T1 cell lysate
Lane 9 : YT cell lysate
Lysates/proteins at 150 µg per lane.
Predicted band size: 72 kDa
Immunocytochemical analysis of Hek-V5-c-Myb transfected cells cytospins (right image) labeling c-Myb using ab169111. ab169111 does not detect other Myb proteins in Hek-V5-a-Myb transfected cells cytospins (left image) or in Hek-V5-b-Myb transfected cells cytospins (middle image).
ab169111 has not yet been referenced specifically in any publications.