Product nameAnti-c-Myb antibody [EPR718(2)]
See all c-Myb primary antibodies
DescriptionRabbit monoclonal [EPR718(2)] to c-Myb
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human c-Myb aa 600-700. The exact sequence is proprietary.
- Jurkat and MOLT4 cell lysates; Human colon tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. PubMed: 22569827|
|WB||1/1000 - 1/10000. Predicted molecular weight: 72 kDa.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. Ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : MOLT4 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
This product has been referenced in:
- Fan J et al. A novel role of CKIP-1 in promoting megakaryocytic differentiation. Oncotarget 8:30138-30150 (2017). Read more (PubMed: 28404913) »
- Li L et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014). Read more (PubMed: 24847116) »