Recombinant
RabMAb

Recombinant Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 647) (ab196932)

Overview

  • Product name

    Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 647)
    See all c-Myb primary antibodies
  • Description

    Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human v-Myb + c-Myb (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab196932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

Target

  • Function

    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • Sequence similarities

    Contains 3 HTH myb-type DNA-binding domains.
  • Domain

    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB proto-oncogene transcription factor antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Images

  • ab196932 staining v-Myb + c-Myb  in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196932 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab196932 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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