Product nameAnti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free
See all c-Myb primary antibodies
DescriptionRabbit monoclonal [EP769Y] to c-Myb (phospho S11) - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, ChIP, Dot blotmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues near the N-terminus of human c-Myb.
- IHC-P: human cervical carcinoma tissue.
ab217945 is the carrier-free version of ab45150 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab217945 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
- Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 647) (ab196932)
- Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 488) (ab197507)
- Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 594) (ab207025)
- Anti-c-Myb (phospho S11) antibody [EP769Y] (Alexa Fluor® 568) (ab207028)
- Anti-c-Myb (phospho S11) antibody [EP769Y] - ChIP Grade (ab45150)
Our Abpromise guarantee covers the use of ab217945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 21296997|
|Dot blot||Use at an assay dependent concentration.|
FunctionTranscriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
DomainComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
- Information by UniProt
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labeling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).
ab217945 has not yet been referenced specifically in any publications.