Recombinant Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) - BSA and Azide free
- Suitable for: WB, IHC-P, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free
See all c-Myb primary antibodies -
Description
Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Dot blotmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, Molt-4, and HL-60 cell lysate. HeLa cell lysate untreated and treated with lambda phosphatase. IHC-P: Human cervical carcinoma tissue. IP: MOLT-4.
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General notes
ab217945 is the carrier-free version of ab45150.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP769Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab196932)
- Alexa Fluor® 488 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab197507)
- Alexa Fluor® 594 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab207025)
- Alexa Fluor® 568 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab312933)
- Alexa Fluor® 555 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab313145)
- Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Domain
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4602 Human
- Omim: 189990 Human
- SwissProt: P10242 Human
- Unigene: 606320 Human
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Alternative names
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
Images
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Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labeling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).
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Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab45150, the same antibody clone in a different buffer formulation.
c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg with ab45150 at 1/120 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg
Lane 2: abab45150 IP in MOLT-4 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45150 in MOLT-4 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Fresh lysate should be used to minimize protein degradation. Multi-bands should be c-myb isoforms based on immunogen design and Uniprot database.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab217945 has been referenced in 1 publication.
- Sun B et al. Establishment of patient-derived xenograft models of adenoid cystic carcinoma to assess pre-clinical efficacy of combination therapy of a PI3K inhibitor and retinoic acid. Am J Cancer Res 11:773-792 (2021). PubMed: 33791153