Recombinant Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP769Y] to c-Myb (phospho S11)
- Suitable for: WB, IHC-P, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-c-Myb (phospho S11) antibody [EP769Y]
See all c-Myb primary antibodies -
Description
Rabbit monoclonal [EP769Y] to c-Myb (phospho S11) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Dot blotmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, Molt-4, and HL-60 cell lysate. HeLa cell lysate untreated and treated with lambda phosphatase. IHC-P: Human cervical carcinoma tissue. IP: MOLT-4.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP769Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab196932)
- Alexa Fluor® 488 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab197507)
- Alexa Fluor® 594 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab207025)
- Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (ab217945)
- Alexa Fluor® 568 Anti-c-Myb (phospho S11) antibody [EP769Y] (ab312933)
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 72 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/50.
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Dot blot |
1/1000.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 72 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/50. |
Dot blot
1/1000. |
Target
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Function
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
Sequence similarities
Contains 3 HTH myb-type DNA-binding domains. -
Domain
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4602 Human
- Omim: 189990 Human
- SwissProt: P10242 Human
- Unigene: 606320 Human
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Alternative names
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
Images
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All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)
Lane 1 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysate with c-Myb (phospho S11) peptide
Lane 2 : Molt-4 cell lysate with c-Myb (phospho S12) peptide
Lane 3 : Molt-4 cell lysate with c-Myb (phospho S11/12) peptide
Lane 4 : Molt-4 cell lysate with c-Myb unmodified peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human vervical carcinoma tissue staining c-Myb with unpurified ab45150.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot blot analysis of c-Myb (phospho S11/12) peptide (Lane 1) and c-Myb unmodified peptide (Lane 2) labeling c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate with c-Myc (phospho S11) peptide
Lane 2 : HeLa cell lysate with c-Myc (phospho S12) peptide
Lane 3 : HeLa cell lysate with c-Myc (phospho S11/12) peptide
Lane 4 : HeLa cell lysate with c-Myc unmodified peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)
Lane 1 : Untreated Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Ramos cell lysate, membrane treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)
Lane 1 : Untreated HL-60 (Human promyelocytic leukemia cell line) cell lysate
Lane 2 : HL-60 cell lysate, membrane treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)
Lane 1 : HeLa cell lysate, the membrane treated with lambda phosphatase
Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/10000 dilution (unpurified) + Ramos (Human Burkitt's lymphoma cell line) membrane lysate
Predicted band size: 72 kDa
Observed band size: 72,75 kDa why is the actual band size different from the predicted? -
c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg with ab45150 at 1/120 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg
Lane 2: ab45150 IP in MOLT-4 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45150 in MOLT-4 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Fresh lysate should be used to minimize protein degradation. Multi-bands should be c-myb isoforms based on immunogen design and Uniprot database.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (57)
ab45150 has been referenced in 57 publications.
- Smith C et al. TAL1 activation in T-cell acute lymphoblastic leukemia: a novel oncogenic 3' neo-enhancer. Haematologica 108:1259-1271 (2023). PubMed: 36632736
- Ji J et al. Distinct clinicopathological and genomic features in solid and basaloid adenoid cystic carcinoma of the breast. Sci Rep 12:8504 (2022). PubMed: 35590093
- Eagle K et al. Transcriptional Plasticity Drives Leukemia Immune Escape. Blood Cancer Discov 3:394-409 (2022). PubMed: 35709529
- Takao S et al. Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia. Elife 10:N/A (2021). PubMed: 33527899
- Sun B et al. Establishment of patient-derived xenograft models of adenoid cystic carcinoma to assess pre-clinical efficacy of combination therapy of a PI3K inhibitor and retinoic acid. Am J Cancer Res 11:773-792 (2021). PubMed: 33791153