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    c-myc-antibody-8-ab17355.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
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Anti-c-Myc antibody [8] (ab17355)

  • Datasheet
Reviews (5) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [8] (ab17355)

    Key features and details

    • Mouse monoclonal [8] to c-Myc
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Recombinant human c-Myc protein (Active) (ab169901)
    ChIP
    Normal Mouse IgG (ab188776)
    Primary
    Product image
    HRP Anti-c-Myc antibody (ab19312)

    View more associated products

    Overview

    • Product name

      Anti-c-Myc antibody [8]
      See all c-Myc primary antibodies
    • Description

      Mouse monoclonal [8] to c-Myc
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Recombinant full length protein corresponding to Human c-Myc.

    • Positive control

      • IHC-P: Human kidney tissue. IP: Human ES cells.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.08% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      8
    • Isotype

      IgG2a
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH / Leucine Zipper
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Nuclear
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Cancer
      • Tumor biomarkers
      • Oncoproteins

    Associated products

    • Assay kits

      • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
    • ChIP Related Products

      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant human c-Myc protein (Active) (ab169901)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17355 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration.

    Target

    • Function

      Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
    • Involvement in disease

      Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
      Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications

      Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
      Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
    • Cellular localization

      Nucleus > nucleoplasm. Nucleus > nucleolus.
    • Target information above from: UniProt accession P01106 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4609 Human
      • Entrez Gene: 17869 Mouse
      • Entrez Gene: 24577 Rat
      • Omim: 190080 Human
      • SwissProt: P01106 Human
      • SwissProt: P01108 Mouse
      • SwissProt: P09416 Rat
      • Unigene: 202453 Human
      • Unigene: 2444 Mouse
      • Unigene: 12072 Rat
      see all
    • Form

      c-Myc is also expressed in the cytoplasm.
    • Alternative names

      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MGC105490 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • myca antibody
      • MYCC antibody
      • Myelocytomatosis oncogene a antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • oncogene c-Myc antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • OTTHUMP00000227763 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      • zc-myc antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [8] (ab17355)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [8] (ab17355)

      Immunohistochemistry of kidney tissue using ab17355 at 10 μg/ml. 

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab17355? Please let us know so that we can cite the reference in this datasheet.

    ab17355 has been referenced in 4 publications.

    • Guan F  et al. New Molecular Mechanism Underlying Myc-Mediated Cytochrome P450 2E1 Upregulation in Apoptosis and Energy Metabolism in the Myocardium. J Am Heart Assoc 8:e009871 (2019). PubMed: 30563421
    • Kosalai ST  et al. EZH2 upregulates the PI3K/AKT pathway through IGF1R and MYC in clinically aggressive chronic lymphocytic leukaemia. Epigenetics 14:1125-1140 (2019). PubMed: 31216925
    • Ivanov AA  et al. OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene 36:5852-5860 (2017). IP . PubMed: 28628118
    • Varlakhanova N  et al. Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells. Epigenetics Chromatin 4:20 (2011). ChIP, ChIP/Chip, IP ; Human . PubMed: 22053792

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-c-Myc antibody [8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Total protein in input
    250 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    MEF
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-c-Myc antibody [8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Permeabilization
    Yes - Triton-X 100
    Specification
    HeLa cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 01 2021

    ELISA abreview for Anti-c-Myc antibody [8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (MYC protein ab169901)
    Specification
    MYC protein ab169901
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 25 2020

    Immunoprecipitation abreview for Anti-c-Myc antibody [8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (PANC-1 cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    PANC-1 cells
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2020

    Western blot abreview for Anti-c-Myc antibody [8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PANC-1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    20 µg
    Treatment
    MYC siRNA (10 nM), 72 hrs
    Specification
    PANC-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 27 2020

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