Anti-c-Myc antibody [8] (ab17355)
- Datasheet
- References (2)
- Protocols
Overview
-
Product nameAnti-c-Myc antibody [8]
See all c-Myc primary antibodies -
DescriptionMouse monoclonal [8] to c-Myc
-
Host speciesMouse
-
Tested applicationsSuitable for: ChIP, ChIP/Chip, IP, WBmore details
-
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Bird
-
Immunogen
Recombinant full length protein (Human).
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A/G purified
-
ClonalityMonoclonal
-
Clone number8
-
IsotypeIgG2a
-
Research areas
Associated products
-
Assay kits
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab17355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use at an assay dependent concentration. PubMed: 22053792Use 3 µg of antibody | |
| ChIP/Chip | Use at an assay dependent concentration. PubMed: 22053792Use 3 µg of antibody | |
| IP | Use at an assay dependent dilution. Suitable with and without SDS | |
| WB | Use at an assay dependent dilution. Detects a band of approximately 64, 67 kDa (predicted molecular weight: 49 kDa). The 67 kDa species is phospho myc. |
Target
-
FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
-
Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
-
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
- Information by UniProt
-
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all -
Formc-Myc is also expressed in the cytoplasm.
-
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Images
-
![Immunoprecipitation - Anti-c-Myc antibody [8] (ab17355)](https://www.abcam.com/ps/products/17/ab17355/Images/c-Myc-Primary-antibodies-ab17355-2.jpg)
Immunoprecipitation - Anti-c-Myc antibody [8] (ab17355)Image from Varlakhanova N et al., Epigenetics Chromatin. 2011 Nov 4;4:20. doi: 10.1186/1756-8935-4-20.; Fig S4.; 4 November 2011, Epigenetics & Chromatin 2011, 4:20Immunoprecipitation analysis of H9 Human ES cells, using ab17355 to immunoprecipitate c-Myc.
Cells were lysed in lysis buffer before being subjected to immunoprecipitation using the primary antibody overnight. Immunocomplexes were recovered with protein-G-sepharose beads. Samples were resolved by SDS-PAGE before transfer to PVDF membrane. The membranes were incubated with the appropriate antibodies and analysed by western blot.
Datasheets and documents
References
This product has been referenced in:
- Ivanov AA et al. OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene 36:5852-5860 (2017). IP . Read more (PubMed: 28628118) »
- Varlakhanova N et al. Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells. Epigenetics Chromatin 4:20 (2011). ChIP, ChIP/Chip, IP ; Human . Read more (PubMed: 22053792) »
