Product nameAnti-c-Myc antibody
See all c-Myc primary antibodies
DescriptionRabbit polyclonal to c-Myc
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
- 293T cells transfected with Myc tag recombinant protein lysate; HeLa Myc tag transfected lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab152146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000.|
|ICC/IF||1/100 - 1/1000.|
FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
- Information by UniProt
Formc-Myc is also expressed in the cytoplasm.
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
All lanes : Anti-c-Myc antibody (ab152146) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Immunofluorescence analysis of paraformaldehyde fixed HeLa Myc tag transfected cells, labeling Myc tag using ab152146 at 1/2500 dilution (green) and alpha tubulin filaments (red).
Myc antibody Immunoprecipitation of Myc-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using:
1: Input, 15ug of HeLa transfected lysates.
2: normal rabbit IgG or
3: 2.5 ug of ab152146.
The precipitated protein was detected by ab152146 diluted at 1/10000.
ab152146 has not yet been referenced specifically in any publications.