Product nameAnti-c-Myc antibody
See all c-Myc primary antibodies
DescriptionRabbit polyclonal to c-Myc
SpecificityThis antibody detects endogenous levels of total Myc protein.
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISA, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human c-Myc.
- Purchase matching WB positive control:Recombinant Human c-Myc protein
- Human breast carcinoma tissue; NIH/3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab39688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18948956|
FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
- Information by UniProt
Formc-Myc is also expressed in the cytoplasm.
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
All lanes : Anti-c-Myc antibody (ab39688) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : NIH/3T3 cell extracts with synthesized peptide
Predicted band size: 49 kDa
Observed band size: 49 kDa
Western blot analysis of extracts from NIH/3T3 cells treated with EGF, in the absence (lane 1) and presence (lane 2) of synthesized peptide, using ab39688. 5-30µg of cell extracts loaded per lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreatic cancer tissue sections labeling c-Myc with ab39688 at 1/50 dilution. Tissue was fixed in paraformaldehyde and permeabilized with TBST. Heat mediated antigen retrieval was performed using a citrate buffer. Tissue was blocked in 10% serum for 1 hour at 20°C. A polyclonal goat anti-rabbit biotin conjugated secondary antibody was used at 1/1000 dilution.
ab39688 staining P22 mouse dorsal skin tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 10% serum for 1 hour at RT. The primary antibody was diluted 1/20 and incubated with the sample for 24 hrs at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary in conjunction with streptavidin-Cy3®.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic cancer tissue sections labeling c-Myc with ab39688 at 1/50 dilution. Tissue was fixed in paraformaldehyde and permeabilized with TBST. Heat mediated antigen retrieval was performed using a citrate buffer. Tissue was blocked in 10% serum for 1 hour at 20°C. A polyclonal goat anti-rabbit biotin conjugated secondary antibody was used at 1/1000 dilution.
ab39688, at 1/50 dilution, staining Human c-Myc in breast carcinoma tissue in the absence (left) and presence (right) of blocking peptide by Immunohistochemistry, Paraffin embedded tissue.
This product has been referenced in:
- Minashima T & Kirsch T Annexin A6 regulates catabolic events in articular chondrocytes via the modulation of NF-?B and Wnt/ß-catenin signaling. PLoS One 13:e0197690 (2018). Read more (PubMed: 29771996) »
- Han CL et al. LncRNA H19 contributes to hippocampal glial cell activation via JAK/STAT signaling in a rat model of temporal lobe epilepsy. J Neuroinflammation 15:103 (2018). WB ; Rat . Read more (PubMed: 29636074) »