Key features and details
- Rabbit polyclonal to c-Myc
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-c-Myc antibody
See all c-Myc primary antibodies
DescriptionRabbit polyclonal to c-Myc
SpecificityThis antibody detects endogenous levels of total Myc protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human c-Myc. The antiserum was produced against synthesized non-phosphopeptide derived from human Myc around the phosphorylation site of threonine 358.
Database link: P01106
Notes regarding use with ICC/IF
Some customers have successfully used ab39688 in ICC/IF, however this product is currently only batch tested in western blot and IHC-P. Please contact Abcam Scientific Support for help on selecting the best antibody for your experiment.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab39688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
- Information by UniProt
Formc-Myc is also expressed in the cytoplasm.
- AU016757 antibody
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- bHLHe39 antibody
All lanes : Anti-c-Myc antibody (ab39688) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : NIH/3T3 cell extracts with synthesized peptide
Lysates/proteins at 40 µg per lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Blocking buffer: 5% (w/v) non-fat dry milk in TBST.
Primary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreatic cancer tissue sections labeling c-Myc with ab39688 at 1/50 dilution. Tissue was fixed in paraformaldehyde and permeabilized with TBST. Heat mediated antigen retrieval was performed using a citrate buffer. Tissue was blocked in 10% serum for 1 hour at 20°C. A polyclonal goat anti-rabbit biotin conjugated secondary antibody was used at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic cancer tissue sections labeling c-Myc with ab39688 at 1/50 dilution. Tissue was fixed in paraformaldehyde and permeabilized with TBST. Heat mediated antigen retrieval was performed using a citrate buffer. Tissue was blocked in 10% serum for 1 hour at 20°C. A polyclonal goat anti-rabbit biotin conjugated secondary antibody was used at 1/1000 dilution.
ab39688 staining P22 mouse dorsal skin tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 10% serum for 1 hour at RT. The primary antibody was diluted 1/20 and incubated with the sample for 24 hrs at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary in conjunction with streptavidin-Cy3®.
ab39688, at 1/50 dilution, staining Human c-Myc in breast carcinoma tissue in the absence (left) and presence (right) of blocking peptide by Immunohistochemistry, Paraffin embedded tissue.
ab39688 has been referenced in 64 publications.
- Luo M & Liang C LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p. Biol Res 53:14 (2020). PubMed: 32293550
- Qian CS et al. MYC-regulated lncRNA NEAT1 promotes B cell proliferation and lymphomagenesis via the miR-34b-5p-GLI1 pathway in diffuse large B-cell lymphoma. Cancer Cell Int 20:87 (2020). PubMed: 32206038
- Sithole N et al. DDX5 potentiates HIV-1 transcription as a co-factor of Tat. Retrovirology 17:6 (2020). PubMed: 32228614
- Wang Y et al. Oxymatrine Inhibits Proliferation and Migration of Vulvar Squamous Cell Carcinoma Cells via Attenuation of the RAS/RAF/MEK/ERK Pathway. Cancer Manag Res 12:2057-2067 (2020). PubMed: 32256113
- Zhang C et al. Silencing of Synuclein-? inhibits human cervical cancer through the AKT signaling pathway. Cell Mol Biol Lett 24:49 (2019). PubMed: 31333726