Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)
![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)](https://www.abcam.com/ps/products/78/ab78318/Images/ab78318-394620-Immunocytochemistry-Immunofluorescence--AnticMyc-phospho-S62-antibody-33A12E10-ab78318.png "Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)")
Key features and details
- Mouse monoclonal [33A12E10] to c-Myc (phospho S62)
- Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-c-Myc (phospho S62) antibody [33A12E10]
See all c-Myc primary antibodies -
Description
Mouse monoclonal [33A12E10] to c-Myc (phospho S62) -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human c-Myc.
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Positive control
- c-Myc siRNA transfected crude cell extracts of AGS (gastric adenocarcinoma) cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
33A12E10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| IHC-P |
Human
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| WB |
Human
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| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.5 - 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa).
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.5 - 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa). |
Target
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all -
Form
c-Myc is also expressed in the cytoplasm. -
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)
Proximity Ligation Analysis with anti-cMyc pS62 and CIP2A antibodies, association of cMyc pS62 with CIP2A (red) in nuclei (DAPI, blue) of HeLa cells by ICC/IF.
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![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)](https://www.abcam.com/ps/products/78/ab78318/Images/ab78318-394618-Immunocytochemistry-Immunofluorescence--AnticMyc-phospho-S62-antibody-33A12E10-ab78318.png)
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)Ab78318 staining (green) and DAPH staining (blue) of c-Myc (phospho S62) in HeLa cells by ICC/IF.
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![Western blot - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)](https://www.abcam.com/ps/products/78/ab78318/Images/ab78318_1.jpg)
Western blot - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)All lanes : Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318) at 1 µg/ml
Lane 1 : Crude cell extracts of AGS (gastric adenocarcinoma) cells with Scr (scrambled) siRNA introduced into the cells as a negative control
Lane 2 : Crude cell extracts of AGS (gastric adenocarcinoma) cells transfected with a negative control siRNA
Lane 3 : Crude cell extracts of AGS (gastric adenocarcinoma) cells transfected with siRNA for c-Myc
Predicted band size: 49 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)](https://www.abcam.com/ps/products/78/ab78318/Images/c-Myc-Primary-antibodies-ab78318-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)IHC image of ab78318 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78318, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)](https://www.abcam.com/ps/products/78/ab78318/Images/c-Myc-Primary-antibodies-ab78318-3.jpg)
Flow Cytometry - Anti-c-Myc (phospho S62) antibody [33A12E10] (ab78318)Overlay histogram showing HL60 cells stained with ab78318 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78318, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed in 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References (18)
ab78318 has been referenced in 18 publications.
- Zhang L et al. Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression. Nat Commun 9:1047 (2018). WB . PubMed: 29535359
- Dingar D et al. MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability. Nat Commun 9:3502 (2018). PubMed: 30158517
- Su Y et al. Post-translational modification localizes MYC to the nuclear pore basket to regulate a subset of target genes involved in cellular responses to environmental signals. Genes Dev 32:1398-1419 (2018). PubMed: 30366908
- Capizzi M et al. MIR7-3HG, a MYC-dependent modulator of cell proliferation, inhibits autophagy by a regulatory loop involving AMBRA1. Autophagy 13:554-566 (2017). PubMed: 28059583
- Al-Zeer MA et al. Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II. EBioMedicine 23:100-110 (2017). PubMed: 28803120
