Application
Western blot
Sample
Human Purified protein (Recombinant human c-Myc protein)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
1 µg
Specification
Recombinant human c-Myc protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1%
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: ~55 kDa
Additional data
Additional Notes
It also detects non-phosphorylated MYC protein.
Detection of P-MYC (Thr58) by Western blot. Western blot analysis after anti-P-MYC (Thr58) antibody incubation showed detection of P-MYC (Thr58) at ~55KDa in samples that were incubated in presence of ATP during the kinase assay.
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Submitted Oct 02 2018