For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    c-myc-phospho-t58-antibody-epr17923-bsa-and-azide-free-ab236021.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
Share by email
RecombinantRabMAb

Recombinant Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Dot Blot - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
  • Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
  • Flow Cytometry (Intracellular) - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
  • Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra), Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 PE Unconjugated

You may also be interested in

Protein
Recombinant human c-Myc protein (Active) (ab169901)
Conjugation
Product image
APC Conjugation Kit - Lightning-Link® (ab201807)
Knockout
Product image
Human MYC (c-Myc) knockout HEK-293T cell line (ab256500)

View more associated products

Overview

  • Product name

    Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free
    See all c-Myc primary antibodies
  • Description

    Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt (Intra), Dot blotmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate, HeLa cells treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes whole cell lysate. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    ab236021 is the carrier-free version of ab185655.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17923
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Alternative Versions

    • Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)
    • PE Anti-c-Myc (phospho T58) antibody [EPR17923] (ab237249)
    • Alexa Fluor® 488 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab300546)
    • Alexa Fluor® 647 Anti-c-Myc (phospho T58) antibody [EPR17923] (ab300731)
  • Assay kits

    • c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.

Target

  • Function

    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in disease

    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4609 Human
    • Entrez Gene: 17869 Mouse
    • Entrez Gene: 24577 Rat
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • SwissProt: P01108 Mouse
    • SwissProt: P09416 Rat
    • Unigene: 202453 Human
    • Unigene: 2444 Mouse
    • Unigene: 12072 Rat
    see all
  • Form

    c-Myc is also expressed in the cytoplasm.
  • Alternative names

    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Cellular myelocytomatosis oncogene antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MGC105490 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • myca antibody
    • MYCC antibody
    • Myelocytomatosis oncogene a antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • oncogene c-Myc antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • OTTHUMP00000227763 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    • zc-myc antibody
    see all

Images

  • Dot Blot - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
    Dot Blot - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

    Lane 1: c-Myc (phospho T58).
    Lane 2: c-Myc (pT58) non-phospho peptide.
    Lane 3: c-Myc (pS62) phospho peptide.
    Lane 4: c-Myc (pS62) non-phospho peptide.

    Dot blot analysis using ab185655 at a dilution of 1/1000. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
    Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling c-Myc (phospho T58) with ab185655 at 1/250, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green).

    Confocal image showing nuclear staining on HeLa cells. The staining decreased after blocking with phospho peptide (100μg/ml) overnight.  The control peptide is a non-phospho peptide.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution Goat Anti-Mouse IgG   (AlexaFluor®594) preadsorbed (ab150120) at 1/500 (red).

    The negative controls are as follows:-
    -ve control 1 -  ab185655 at 1/500  followed by ab150120  at 1/500.
    -ve control 2 -ab7291 at 1/1000  followed by ab150077  at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185655).

  • Flow Cytometry (Intracellular) - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
    Flow Cytometry (Intracellular) - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

    This data was developed using ab185655, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 min cells labeling c-Myc with purified ab185655 at 1/700 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
    Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab236021? Please let us know so that we can cite the reference in this datasheet.

ab236021 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab236021.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.