Recombinant Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free
See all c-Myc primary antibodies -
Description
Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human c-Myc aa 50-150 (phospho T58). The exact sequence is proprietary.
Database link: P01106 -
Positive control
- ICC/IF: HeLa cells.
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General notes
Ab236021 is the carrier-free version of ab185655. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab236021 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17923 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab236021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa). |
Target
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all -
Form
c-Myc is also expressed in the cytoplasm. -
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)](https://www.abcam.com/ps/products/236/ab236021/Images/ab236021-311961-23602122.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] - BSA and Azide free (ab236021)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling c-Myc (phospho T58) with ab185655 at 1/250, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green).
Confocal image showing nuclear staining on HeLa cells. The staining decreased after blocking with phospho peptide (100μg/ml) overnight. The control peptide is a non-phospho peptide.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/500 (red).
The negative controls are as follows:-
-ve control 1 - ab185655 at 1/500 followed by ab150120 at 1/500.
-ve control 2 -ab7291 at 1/1000 followed by ab150077 at 1/500.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185655).
Datasheets and documents
References
ab236021 has not yet been referenced specifically in any publications.
