For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    c-myc-transcription-factor-assay-kit-colorimetric-ab207200.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
Share by email

c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for c-Myc activation. These results are provided for demonstration purposes only.

    Key features and details

    • Assay type: Semi-quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 3 hr 30 min
    • Sample type: Nuclear Extracts
    • Sensitivity: 250 ng/well

    You may also be interested in

    Biochemical
    Product image
    10058-F4, c-Myc-Max dimerization inhibitor (ab145065)
    Assay
    Product image
    Total CREB Transcription Factor Assay Kit (Colorimetric) (ab207201)
    Preparation
    Product image
    Catalase beads (ab218718)

    View more associated products

    Overview

    • Product name

      c-Myc Transcription Factor Assay Kit (Colorimetric)
      See all c-Myc kits
    • Detection method

      Colorimetric
    • Sample type

      Nuclear Extracts
    • Assay type

      Semi-quantitative
    • Sensitivity

      < 250 ng/well
    • Assay time

      3h 30m
    • Species reactivity

      Reacts with: Mouse, Human
    • Product overview

      c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200) is a high throughput assay to quantify c-Myc activation in nuclear extracts. This assay combines a quick ELISA format with a sensitive and specific non-radioactive assay for transcription factor activation.


      A specific double stranded DNA sequence containing the c-Myc consensus binding site (5’ –CACGTG– 3’) has been immobilized onto a 96-well plate. Active c-Myc present in the nuclear extract specifically binds to the oligonucleotide. c-Myc is detected by a primary antibody that recognizes an epitope of c-Myc accessible only when the protein is activated and bound to its target DNA. An HRP-conjugated secondary antibody provides sensitive colorimetric readout that at OD 450 nm. This product detects human and mouse c-Myc.


      Key performance and benefits:



      • Assay time: 3.5 hours (cell extracts preparation not included).

      • Detection limit: < 0.25 µg nuclear extract/well.

      • Detection range: 0.25 – 5 µg nuclear extract/well.

    • Notes

      c-Myc is a transcription factor that regulates cell growth and differentiation, glycolysis and apoptosis and deregulation of c-Myc has been implicated in the origin of diverse human cancers.

      c-Myc was originally discovered as the cellular homolog of the retroviral v-myc oncogene, and is a transcription factor involved in a wide variety of cellular processes, including cell proliferation, replicative potential, growth, differentiation and apoptosis. Expression of c-Myc is induced by mitogenic signals and suppressed by growth-inhibitory signals. c-Myc is a member of the basic helix-loop-helix leucine zipper (bHLHzip) family, along with B-Myc, N-Myc, L-Myc and s-Myc. Upon dimerization with the bHLHzip protein Max, c-Myc can bind to the E box motif CACGTG and activate transcription. c-Myc is phosphorylated at Ser62, which has been shown to be a regulatory site of phosphorylation. The phosphorylation of c-Myc causes increased function of the NH2-terminal transactivation domain, and studies have indicated that the expression of MAP kinase is responsible for increased c-Myc phosphorylation at Ser62.

      Because of the central role of c-Myc as an activator of diverse cellular processes, regulation of this transcription factor is crucial for proper cell function and ultimate survival. The main regulation of c-Myc occurs through its binding with the bHLHzip protein Max, which can also form heterodimers with members of the Mad family (Mad 1, 3, 4 and Mxi1). As c-Myc cannot bind to DNA without forming a heterodimer with Max, competition between c-Myc and Mad for the common partner Max is used to regulate c-Myc activity. While Max is a relatively stable protein, c-Myc degrades rapidly, with a half-life of 20-30 minutes.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 x 96 tests 5 x 96 tests
      10X Antibody Binding Buffer 1 x 2.2ml 5 x 2.2ml
      10X Wash Buffer 1 x 22ml 5 x 22ml
      96-well c-Myc assay plate 1 unit 5 units
      Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 5 x 11µl
      Binding Buffer 1 x 10ml 5 x 10ml
      c-Myc antibody 1 x 11µl 5 x 11µl
      c-Myc Mutated oligonucleotide (10 pmol/μL) 1 x 100µl 5 x 100µl
      c-Myc Wild-type oligonucleotide (10 pmol/μL) 1 x 100µl 5 x 100µl
      Developing Solution 1 x 11ml 5 x 11ml
      Dithiothreitol (DTT) (1 M) 1 x 100µl 5 x 100µl
      Jurkat (1 day growth) nuclear extract (2.5 μg/μL) 1 x 40µl 5 x 40µl
      Lysis Buffer 1 x 10ml 5 x 10ml
      Plate sealer 1 unit 5 units
      Protease Inhibitor Cocktail 1 x 100µl 5 x 100µl
      Stop Solution 1 x 11ml 5 x 11ml
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH / Leucine Zipper
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Nuclear
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Cancer
      • Tumor biomarkers
      • Oncoproteins
    • Function

      Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
    • Involvement in disease

      Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
      Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications

      Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
      Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
    • Cellular localization

      Nucleus > nucleoplasm. Nucleus > nucleolus.
    • Target information above from: UniProt accession P01106 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • AU016757
      • Avian myelocytomatosis viral oncogene homolog
      • bHLHe39
      • c Myc
      • Cellular myelocytomatosis oncogene
      • Class E basic helix-loop-helix protein 39
      • MGC105490
      • MRTL
      • Myc
      • Myc protein
      • Myc proto oncogene protein
      • Myc proto-oncogene protein
      • myc-related translation/localization regulatory factor
      • MYC_HUMAN
      • Myc2
      • myca
      • MYCC
      • Myelocytomatosis oncogene
      • Myelocytomatosis oncogene a
      • Niard
      • Nird
      • oncogene c-Myc
      • Oncogene Myc
      • OTTHUMP00000158589
      • OTTHUMP00000227763
      • Proto-oncogene c-Myc
      • Protooncogene homologous to myelocytomatosis virus
      • RNCMYC
      • Transcription factor p64
      • Transcriptional regulator Myc-A
      • V-Myc avian myelocytomatosis viral oncogene homolog
      • v-myc myelocytomatosis viral oncogene homolog (avian)
      • zc-myc
      see all
    • Database links

      • Entrez Gene: 4609 Human
      • Entrez Gene: 17869 Mouse
      • Omim: 190080 Human
      • SwissProt: P01106 Human
      • SwissProt: P01108 Mouse
      • Unigene: 202453 Human
      • Unigene: 2444 Mouse

      Associated products

      • Related Products

        • MAPK (ATF2, c-Jun, c-Myc, MEF2, STAT1alpha) Transcription Factor Assay Kit (Colorimetric) (ab207212)

      Images

      • Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for c-Myc activation. These results are provided for demonstration purposes only.
        Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for c-Myc activation. These results are provided for demonstration purposes only.

        Different amounts of unstimulated U-937 (grey) and Jurkat (black) were tested for c-Myc activation. These results are provided for demonstration purposes only.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab207200? Please let us know so that we can cite the reference in this datasheet.

      ab207200 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab207200.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.