Overview

  • Product name

    C-Peptide ELISA Kit
    See all C Peptide kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    C-peptide 16 <=6.2%
    Inter-assay
    Sample n Mean SD CV%
    C-peptide 20 <=10%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.01 ng/ml
  • Range

    0.7 ng/ml - 1.9 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    C-peptide ELISA kit (ab178641) is designed for the accurate quantitative measurement of C-peptide in Human serum and plasma.


    A 96-well plate has been precoated with Streptavidin. Samples, standards and the C-peptide HRP and Biotin conjugate are added to the wells. Biotinylated monoclonal and horseradish peroxidase (HRP) labelled antibodies are added and the reactants are mixed. The different types of antibodies used have high affinity and specificity and are directed against distinct and different epitopes of C-Peptide. Reaction between the various C-Peptide antibodies and native C-Peptide occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of C-peptide in the sample and the intensity is measured at 450 nm.

  • Notes

    C-peptide is the abbreviation for connecting peptide; it is a 31-amminoacid peptide. C-peptide of insulin is the C-terminal cleavage product produced during processing of the insulin pro-hormone to the mature insulin molecule. Proinsulin is cleaved when it is released from the pancreas into the blood - one C-peptide for each insulin molecule. C-Peptide is devoid of any biological activity but appears to be necessary to maintain the structural integrity of Insulin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    50X Washing Solution 1 x 20ml
    Cover Foil 1 unit
    C-Peptide HRP and Biotin Conjugate 1 x 13ml
    C-Peptide Standard 0 - 0 ng/mL (Lyophilized) 1 vial
    C-Peptide Standard 1 - 0.2 ng/mL (Lyophilized) 1 vial
    C-Peptide Standard 2 - 1.0 ng/mL (Lyophilized) 1 vial
    C-Peptide Standard 3 - 2.0 ng/mL (Lyophilized) 1 vial
    C-Peptide Standard 4 - 5.0 ng/mL (Lyophilized) 1 vial
    C-Peptide Standard 5 - 10.0 ng/mL (Lyophilized) 1 vial
    Stop Solution 1 x 15ml
    Streptavidin Coated Microplate (12 x 8 wells) 1 unit
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Hyperproinsulinemia, familial
    Diabetes mellitus, insulin-dependent, 2
    Diabetes mellitus, permanent neonatal
    Maturity-onset diabetes of the young 10
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-peptide
    • ins
    • INS_HUMAN
    • Insulin A chain
  • Database links

Applications

Our Abpromise guarantee covers the use of ab178641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab178641.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up