Overview

  • Product name

    Anti-C Reactive Protein antibody - Aminoterminal end
    See all C Reactive Protein primary antibodies
  • Description

    Rabbit polyclonal to C Reactive Protein - Aminoterminal end
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminal of human C Reactive Protein identical to the related rat and mouse sequence.

  • Positive control

    • Rat liver tissue lysate and mouse lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab65842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

Images

  • ab65842 staining C Reactive Protein in mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked for 5 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 1X PBS) for 17 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-C Reactive Protein antibody - Aminoterminal end (ab65842) at 2 µg/ml + rat liver tissue lysate at 30 µg

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

References

This product has been referenced in:

  • Inoue M  et al. Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis. Nat Commun 9:5116 (2018). Read more (PubMed: 30504805) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab46819.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up