Anti-C Reactive Protein antibody [C5] (ab8279)

Reviews (1)Q&A (5)References (5)

Overview

  • Product name

    Anti-C Reactive Protein antibody [C5]
    See all C Reactive Protein primary antibodies

  • Description

    Mouse monoclonal [C5] to C Reactive Protein

  • Host species

    Mouse

  • Specificity

    Human CRP Specific antibody titer in ELISA is > 10 to the power of -6

  • Tested applications

    Suitable for: ELISA, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) (Human).

  • General notes

    Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab31156 or ab50861 as possible replacements.

    Detects CRP in the presence and absence of Ca2+.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab8279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [C6] to C Reactive Protein (ab8278). Capture
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [C6] to C Reactive Protein (HRP) (ab24462).

Target

  • Function

    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

  • Tissue specificity

    Found in plasma.

  • Sequence similarities

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    References

    This product has been referenced in:

    • Ouyang M & Di Carlo D Nanoplasmonic swarm biosensing using single nanoparticle colorimetry. Biosens Bioelectron 132:162-170 (2019). Read more (PubMed: 30875628) »
    • Loukov D  et al. Monocyte activation is elevated in women with knee-osteoarthritis and associated with inflammation, BMI and pain. Osteoarthritis Cartilage 26:255-263 (2018). Read more (PubMed: 29128509) »
    See all 5 Publications for this product

    Publishing research using ab8279? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    ELISA abreview for Anti-C Reactive Protein antibody [C5]

     Good
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Serum (blood)
    Specification
    blood
    Blocking step
    SuperBlock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Type
    Sandwich (Capture)
    Read More

    Mr. Falak Iqbal

    Verified customer

    Submitted Oct 18 2017

    Question

    I am planning to test a C-reactive protein sandwich ELISA assay and was hoping that you could suggest the most suitable antibody pair for this application from your range?


    Also, on your website it lists the molecular weight of the C-Reactive Protein subunit as being 23 kDa, but also states that the protein forms a pentamer in solution. Please can you advise on whether in a solution ELISA assay I would be detecting the subunits alone (23 kDa) or the pentameric form (115000 kDa)?

    Read More
    Answer


    I have confirmed with the lab and most of our C Reactive Protein antibodies, except C3 and C4, react with both monomer and pentamer form of CRP. (As our clones C3 and C4 do not recognize CRP in the absence of Ca2+ ions, we suggest that they could not recognize CRP monomer, but we have never experimentally tested this.)


    ab10026, Anti-C Reactive Protein antibody [C1]


    ab136176, Anti-C Reactive Protein antibody [C2]


    ab8279, Anti-C Reactive Protein antibody [C5]


    ab24462, Anti-C Reactive Protein antibody [C6] (HRP)


    As pair I can recommend ab24462 and ab8279 to recognize both, monomer and pentamer.


    https://www.abcam.com/index.html?datasheet=24462 (or use the following: https://www.abcam.com/index.html?datasheet=24462).


    https://www.abcam.com/index.html?datasheet=8279 (or use the following: https://www.abcam.com/index.html?datasheet=8279).

    Read More

    Question

    Dear technical team,

    Our customer is going to purchase ab24462 as detection antibody of sELISA.
    She would like to receive data that ab8280 or ab8279 used as capture antibody together because she concerns if they are working well.
    Please let me know if you have information.

    Best regards

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab24462 can be paired for ELISA with https://www.abcam.com/index.html?datasheet=8279 and https://www.abcam.com/index.html?datasheet=8280 so our Abguarantee applies to these products. I regret to inform you that currentlywe are unable to send you data.

    Apologies!

    Read More

    Question

    Dear technical team,

    Our customer is going to purchase ab24462 as detection antibody of sELISA.
    She would like to receive data that ab8280 or ab8279 used as capture antibody together because she concerns if they are working well.
    Please let me know if you have information.

    Best regards

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab24462 can be paired for ELISA with https://www.abcam.com/index.html?datasheet=8279 and https://www.abcam.com/index.html?datasheet=8280 so our Abguarantee applies to these products. Unfortunately, we are unable to send you testing data butI would like to reassure your customer that ourguarantee covers these products for sELISA.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    We are currently looking for a good pairing for C-Reactive protein antibodies and wonder if you guys could recommend any pair of antibodies? The antibodies will be used in sandwich ELISA and we are looking for antibodies which are already paired by Abcam for optimal binding and lowest cross reactivity of capture and detection antibody.
    We've noticed that it is recommended to pair antibodies according to their C number, for example C2-C6, C5-C6 and C7-C6, information found on website: https://www.abcam.com/C-Reactive-Protein-antibody-C6-ab8278.html.
    It is best to pair antibodies to their C numbers as mentioned?

    Looking forward to your reply.


    Thanks and regards,

    Read More
    Answer

    Thank you for contacting us.
    We do not use the terminology C number. These does not depicts any information other the clone numbers for a particular antibody.
    The sELISA pairs we recommend are
    The best pair is C2-C6 (ab24461 detection - ab8278 capture)
    C5-C6 (ab8279 capture - ab24462 detection)
    C7-C6 (ab8280 capture - ab24462 detection)
    Please ab24462 is a HRP conjugated anti C reactive protein antibody; due to the conjugation it will be a good detection antibody.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Please could you tell me if these antibodies are influenced in any way by the presence of calcium? Thank you.

    Read More
    Answer

    Our antibodies to CRP (clones C2 and C6) are strongly specific to CRP molecule and form a unique two-site combination for the development of fast and sensitive CRP assay. Mabs are not sensitive to the presence of Ca2+- binding reagents in the sample and can be used for quantitative CRP detection in EDTA or citrate plasma samples. Additional information can be found from www.hytest.fi.

    Read More

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