Overview

  • Product name
    Anti-C Reactive Protein antibody [C6] (HRP)
    See all C Reactive Protein primary antibodies
  • Description
    Mouse monoclonal [C6] to C Reactive Protein (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Specificity
    Human CRP
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human)

  • General notes
    Specific antibody titer in ELISA is > 10 to the power of -6

Properties

Applications

Our Abpromise guarantee covers the use of ab24462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [C5] to C Reactive Protein (ab8279) and Mouse monoclonal [C7] to C Reactive Protein (ab8280).



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • Tissue specificity
      Found in plasma.
    • Sequence similarities
      Belongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    References

    This product has been referenced in:
    • Verschoor CP  et al. DNA methylation patterns are related to co-morbidity status and circulating C-reactive protein levels in the nursing home elderly. Exp Gerontol 105:47-52 (2018). Sandwich ELISA . Read more (PubMed: 29032243) »
    • Skogstrand K  et al. Effects of blood sample handling procedures on measurable inflammatory markers in plasma, serum and dried blood spot samples. J Immunol Methods 336:78-84 (2008). Read more (PubMed: 18495149) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer


    I have confirmed with the lab and most of our C Reactive Protein antibodies, except C3 and C4, react with both monomer and pentamer form of CRP. (As our clones C3 and C4 do not recognize CRP in the absence of Ca2+ ions, we suggest that they could not recognize CRP monomer, but we have never experimentally tested this.)


    ab10026, Anti-C Reactive Protein antibody [C1]


    ab136176, Anti-C Reactive Protein antibody [C2]


    ab8279, Anti-C Reactive Protein antibody [C5]


    ab24462, Anti-C Reactive Protein antibody [C6] (HRP)


    As pair I can recommend ab24462 and ab8279 to recognize both, monomer and pentamer.


    https://www.abcam.com/index.html?datasheet=24462 (or use the following: https://www.abcam.com/index.html?datasheet=24462).


    https://www.abcam.com/index.html?datasheet=8279 (or use the following: https://www.abcam.com/index.html?datasheet=8279).

    Read More

    Answer

    Thank you for your enquiry.

    I can confirm that ab24462 can be paired for ELISA with https://www.abcam.com/index.html?datasheet=8279 and https://www.abcam.com/index.html?datasheet=8280 so our Abguarantee applies to these products. I regret to inform you that currentlywe are unable to send you data.

    Apologies!

    Read More

    Answer

    Thank you for your enquiry.

    I can confirm that ab24462 can be paired for ELISA with https://www.abcam.com/index.html?datasheet=8279 and https://www.abcam.com/index.html?datasheet=8280 so our Abguarantee applies to these products. Unfortunately, we are unable to send you testing data butI would like to reassure your customer that ourguarantee covers these products for sELISA.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    The combination of biotinylated ab8280 and HRP-conjugated ab24462 should work, as these two clones have been tested together and are known to be an effective pair for sandwich ELISA. The goat polyclonal ab19174 has not been tested with ab8280. It may work but I think you are better off with ab8280 combined with ab24462. In addition, the FITC conjugate will require a fluorescent plate reader, in case you were not already aware of that.

    Read More

    Answer

    Thank you for contacting. My search also shows just the single biotinylated antibody, anti-rat CRP, ab86246.

    We do have biotinylation kits which are very easy to use. For instance, the rabbit polyclonal antibody ab31156 can be biotinylated with ab102863 or ab102866, depending on the intended use of the antibody. (Please see the Product Overview section of each datasheet for an explanation of the differences between the two products).

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=86246).

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=102863).

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=102866).

    Please let me know if you have any questions.

    Read More

    Answer

    Thank you for contacting us.
    We do not use the terminology C number. These does not depicts any information other the clone numbers for a particular antibody.
    The sELISA pairs we recommend are
    The best pair is C2-C6 (ab24461 detection - ab8278 capture)
    C5-C6 (ab8279 capture - ab24462 detection)
    C7-C6 (ab8280 capture - ab24462 detection)
    Please ab24462 is a HRP conjugated anti C reactive protein antibody; due to the conjugation it will be a good detection antibody.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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