Recombinant Anti-C Reactive Protein antibody [EPR20063] (ab207756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20063] to C Reactive Protein
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-C Reactive Protein antibody [EPR20063]
See all C Reactive Protein primary antibodies -
Description
Rabbit monoclonal [EPR20063] to C Reactive Protein -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat serum; Rat liver and kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20063 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1401 Human
- Entrez Gene: 12944 Mouse
- Entrez Gene: 25419 Rat
- Omim: 123260 Human
- SwissProt: P02741 Human
- SwissProt: P14847 Mouse
- SwissProt: P48199 Rat
- Unigene: 709456 Human
see all -
Alternative names
- Pentraxin 1, short antibody
- C reactive protein antibody
- C reactive protein pentraxin related antibody
see all
Images
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Lanes 1-2 & 4 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution
Lane 3 : Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/5000 dilution
Lane 1 : Human serum
Lane 2 : Rat liver lysate
Lane 3 : Rat serum
Lane 4 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25,30 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2/4: 30 seconds; Lane 3: 1 second.
Observed MWt: 25 kDa (Human), 30 kDa (Rat-glycosylated).
The molecular weight observed is consistent with what has been described in the literature (PMID: 7093286).
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Anti-C Reactive Protein antibody [EPR20063] (ab207756) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab207756 has been referenced in 1 publication.
- Fu S et al. miR-875-5p regulates IR and inflammation via targeting TXNRD1 in gestational diabetes rats. Mol Med Rep 23:N/A (2021). PubMed: 33649852