Recombinant Anti-C Reactive Protein antibody [Y284] (ab32412)
![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-253925-anti-c-reactive-protein-antibody-y284-western-blot.jpg "Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y284] to C Reactive Protein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C Reactive Protein antibody [Y284]
See all C Reactive Protein primary antibodies -
Description
Rabbit monoclonal [Y284] to C Reactive Protein -
Host species
Rabbit -
Specificity
ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.
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Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available asab216758) -
Epitope
ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein. -
Positive control
- WB: Human serum and plasma lysates. IHC-P: Normal human liver tissue. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y284 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
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| IP |
1/30 - 1/50.
|
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| IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 25 kDa. |
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IP
1/30 - 1/50. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
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Alternative names
- Pentraxin 1, short antibody
- C reactive protein antibody
- C reactive protein pentraxin related antibody
see all
Images
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Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-253883-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-253884-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-253849-anti-c-reactive-protein-antibody-y284-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab32412 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-44736-anti-c-reactive-protein-antibody-y284-western-blot.gif)
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-44735-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.
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![Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-7-benefits-of-recombinant-antibodies.png)
Anti-C Reactive Protein antibody [Y284] (ab32412)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab32412 has been referenced in 31 publications.
- Jiang S et al. Formation of protein cross-links by singlet oxygen-mediated disulfide oxidation. Redox Biol 41:101874 (2021). PubMed: 33601275
- Jiang S et al. Crosslinking of human plasma C-reactive protein to human serum albumin via disulfide bond oxidation. Redox Biol 41:101925 (2021). PubMed: 33714740
- Szekely L et al. Pulmonary stromal expansion and intra-alveolar coagulation are primary causes of COVID-19 death. Heliyon 7:e07134 (2021). PubMed: 34056141
- Jones A et al. Ki-67 "hot spot" digital analysis is useful in the distinction of hepatic adenomas and well-differentiated hepatocellular carcinomas. Virchows Arch N/A:N/A (2020). PubMed: 32583014
- Kim EN et al. High serum CRP influences myocardial miRNA profiles in ischemia-reperfusion injury of rat heart. PLoS One 14:e0216610 (2019). PubMed: 31063484