Anti-C Reactive Protein antibody [Y284] (ab32412)
- Datasheet
- References (19)
- Protocols
Overview
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Product nameAnti-C Reactive Protein antibody [Y284]
See all C Reactive Protein primary antibodies -
DescriptionRabbit monoclonal [Y284] to C Reactive Protein
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Host speciesRabbit
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Specificityab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2.
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available asab216758) -
Epitopeab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
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Positive control
- WB: Human serum and plasma lysates. IHC-P: Normal human liver tissue. IP: HepG2 whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberY284
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.Can be blocked with C Reactive Protein peptide (ab216758). | |
| IP | 1/30 - 1/50. | |
| IHC-P | 1/100 - 1/250. |
Target
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FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
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Tissue specificityFound in plasma.
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Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
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Alternative names
- Pentraxin 1, short antibody
- C reactive protein antibody
- C reactive protein pentraxin related antibody
see all
Images
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![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-6-ab32412WB1.jpg)
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-4-ab32412IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-5-ab32412IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412-3-ab32412IP1.jpg)
Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab32412 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412wb.gif)
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.com/ps/products/32/ab32412/Images/ab32412ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.
References
This product has been referenced in:
- Hanna M et al. Association between physical activity and the expression of mediators of inflammation in normal breast tissue among premenopausal and postmenopausal women. Cytokine 102:151-160 (2018). Read more (PubMed: 29102166) »
- Hanna M et al. Association between local inflammation and breast tissue age-related lobular involution among premenopausal and postmenopausal breast cancer patients. PLoS One 12:e0183579 (2017). Read more (PubMed: 28846716) »
