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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-C Reactive Protein antibody [Y284] (ab32412)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (24)

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Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
  • Anti-C Reactive Protein antibody [Y284] (ab32412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y284] to C Reactive Protein
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

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Anti-liver FABP antibody [EPR20464] (ab222517)

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Overview

  • Product name

    Anti-C Reactive Protein antibody [Y284]
    See all C Reactive Protein primary antibodies
  • Description

    Rabbit monoclonal [Y284] to C Reactive Protein
  • Host species

    Rabbit
  • Specificity

    ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab216758)

  • Epitope

    ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
  • Positive control

    • WB: Human serum and plasma lysates. IHC-P: Normal human liver tissue. IP: HepG2 whole cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y284
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Acute Phase Reactants
    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Heart
    • Apoptosis
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Alternative Versions

    • Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
    • Anti-C Reactive Protein antibody [Y284] - BSA and Azide free (ab271830)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human C Reactive Protein (ab167710)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
IP
1/30 - 1/50.
IHC-P (3)
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
IP
1/30 - 1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • Tissue specificity

      Found in plasma.
    • Sequence similarities

      Belongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02741 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1401 Human
      • Omim: 123260 Human
      • SwissProt: P02741 Human
      • Unigene: 709456 Human
      • Unigene: 76452 Human
      • Alternative names

        • Pentraxin 1, short antibody
        • C reactive protein antibody
        • C reactive protein pentraxin related antibody
        • C-reactive protein(1-205) antibody
        • CRP antibody
        • CRP_HUMAN antibody
        • MGC88244 antibody
        • Pentraxin 1 antibody
        • PTX 1 antibody
        • PTX1 antibody
        see all

      Images

      • Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
        All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)

        Lane 1 : Human serum
        Lane 2 : Human plasma

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

        Predicted band size: 25 kDa
        Observed band size: 27 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      • Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)

        ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.

        Lane 1 (input): HepG2 whole cell lysate (10µg)

        Lane 2 (+): ab32412 + HepG2 whole cell lysate.

        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

        For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum

        Predicted band size: 25 kDa
        Observed band size: 27 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

      • Anti-C Reactive Protein antibody [Y284] (ab32412)
        Anti-C Reactive Protein antibody [Y284] (ab32412)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (24)

      Publishing research using ab32412? Please let us know so that we can cite the reference in this datasheet.

      ab32412 has been referenced in 24 publications.

      • Schlabritz-Loutsevitch N  et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. J Med Primatol 48:68-73 (2019). PubMed: 30246873
      • Kim EN  et al. High serum CRP influences myocardial miRNA profiles in ischemia-reperfusion injury of rat heart. PLoS One 14:e0216610 (2019). PubMed: 31063484
      • Zhang G  et al. Serum amyloid a as an indicator of impending xenograft failure: Experimental studies. Int J Surg 60:283-290 (2018). PubMed: 30521954
      • Ferreira R  et al. Exercise Training Impacts Cardiac Mitochondrial Proteome Remodeling in Murine Urothelial Carcinoma. Int J Mol Sci 20:N/A (2018). PubMed: 30602657
      • Hanna M  et al. Association between physical activity and the expression of mediators of inflammation in normal breast tissue among premenopausal and postmenopausal women. Cytokine 102:151-160 (2018). PubMed: 29102166
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C Reactive Protein antibody [Y284]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Frontal Cortex (Brain))
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA
      Permeabilization
      Yes - .1% TritonX-100
      Specification
      Frontal Cortex (Brain)
      Blocking step
      Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 25 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C Reactive Protein antibody [Y284]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Choroid plexus)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EDTA
      Permeabilization
      No
      Specification
      Choroid plexus
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 22 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-C Reactive Protein antibody [Y284]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Liver)
      Specification
      Liver
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      Submitted May 29 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C Reactive Protein antibody [Y284]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Brain - alzheimer disease)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EDTA pH = 8
      Permeabilization
      No
      Specification
      Brain - alzheimer disease
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 01 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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